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UCheck 7 Drug Adulteration Test Strip

For in vitro use only. Read all instructions, precautions, and
chlorochromate to form a blue color complex. Other colors limitations before performing this test. may indicate the presence of other oxidants. pH: Testing for the presence of acidic or alkaline adulterant.
This test is based on the well-known double pH indicator UCheck 7 is a fast dip-and-read test for the determination of method that gives distinguishable colors over wide pH range. diluted or adulterated urine specimens. It is an important The colors range from orange (low pH) to yellow and green to pre-screening test for any drug-testing program. SUMMARY AND EXPLANATION
Specific Gravity: Testing for sample dilution. This test is
UCheck 7 drug adulteration tests are firm plastic strips to which based on the apparent pKa change of certain pretreated seven different reagent areas are affixed. UCheck 7 test strips polyelectrolytes in relation to the ionic concentration. In the are ready-to-use and disposable. No equipment is required for presence of an indicator, the colors range from dark blue or its use. Only fresh and uncentrifuged urine samples without blue-green in urine of low ionic concentration to green and yellow in urine of higher ionic concentration. UCheck 7 provides tests for Creatinine, Nitrite, pH, Specific Bleach: Testing for the presence of bleach in urine. In this test,
Gravity, Glutaraldehyde, Oxidants, and Pyridium the presence of bleach forms a blue-green color complex. Chlorochromate in urine. Test results may be useful for assessing the integrity of the urine sample prior to Pyridium Chlorochromate: Testing for the presence of
Drugs-of-Abuse testing; for example, whether the sample is chromate in urine. In this test, the presence of chromate forms a possibly diluted with water or other liquids as indicated by the creatinine and specific gravity tests. UCheck 7 detects whether the sample contains commercially available adulterants including nitrite, glutaraldehyde, bleach, pyridinium 1. Store at room temperature between 59– 86 oF (15 – 30 oC). chlorochromate and other oxidizing agents. UCheck 7 can also 2. All test strips should be stored in the original container. assess whether the sample is possibly contaminated by acidic (vinegar) or basic (ammonia solution) adulterants as indicated 4. Remove only as many strips required for testing and immediately recap the container tightly. TEST PRINCIPLE
In general, all seven tests are based on the chemical reactions of the indicator reagents on the pads with components in the urine PRECAUTIONS
sample effecting color changes. Results are obtained by comparing the color on each of the test pads with the corresponding pad on the container color chart label. SPECIMEN COLLECTION
1. Collect urine in a clean glass or plastic container. Creatinine: Testing for sample dilution. In this assay,
2. Test urine sample as soon as possible after collection. creatinine reacts with a creatinine indicator in an alkaline Refrigerate urine sample immediately if the sample cannot condition to form a purplish-brown color complex. The be tested within one hour. Bring refrigerated sample to concentration of creatinine is directly proportional to the color room temperature and mix thoroughly before testing. 3. Do not centrifuge or add preservatives to the urine sample. 4. Handle the urine sample as if it is potentially infectious. Glutaraldehyde: Testing for the presence of exogenous
5. Aliquot a small portion of the urine sample into another aldehyde. In this assay, the aldehyde group on the container for testing in order to avoid contamination of the glutaraldehyde reacts with an indicator to form a pink/purple whole urine sample. Do not dip UCheck 7 directly into the Nitrite: Testing for the presence of exogenous nitrite. Nitrite
reacts with an aromatic amine to form a diazonium compound 1. Remove from the bottle only enough strips for immediate in an acid medium. The diazonium compound in turn couples with an indicator to produce a pink-red/purple color. 2. Completely immerse reagent areas of the strip in fresh, well-mixed urine. Remove the strip immediately to avoid Oxidants: Testing for presence of oxidizing reagents. In this
reaction, a color indicator reacts with oxidants such as 3. While removing, touch the side of the strip against the rim hydrogen peroxide, ferricyanide, persulfate, or pyridinium of the urine container to remove excess urine. Blot the lengthwise edge of the strip on an absorbent paper towel to the possibility of adulteration. However, false positive may further remove excess urine and avoid running over result when ketone bodies are presence in urine. Ketone bodies (contamination from adjacent reagent pads.) may appear in urine when a person is in ketoacidosis, starvation 4. Compare each reagent area to its corresponding color blocks on the color chart and read at the times specified. Proper read time is critical for optimal results. Nitrite: Although nitrite is not a normal component of urine,
5. Obtain results by direct color chart comparison. nitrite levels of up to 3.6 mg/dl may be found in some urine specimens due to urinary tract infections, bacterial
contamination or improper storage.
Oxidants: The presence of oxidizing reagents in the urine is
indicative of adulteration since oxidizing reagents are not
normal constituents of urine. Oxidizing reagents include
Hydrogen Peroxide, Ferricyanide, Persulfate, Pyridinium
Note: All reagent areas may be read between 1 - 2 minutes for pH: Normal urine pH ranges from 4.5 to 8.0. Values below pH
screening positive urine from negative urine. Changes in color 4.0 or above pH 9.0 are indicative of adulteration. after 2 minutes are of no diagnostic value. Specific Gravity: Random urine may vary in specific gravity
from 1.003 - 1.030. Normal adults with normal diets and Semi-quantitative results are obtained by visually comparing normal fluid intake will have an average urine specific gravity the color of each pad with the corresponding test color block of 1.016 - 1.0227. Elevated urine specific gravity value may be pictured on the container label. No equipment is required. obtained in the presence of moderate quantities of protein. DOT guidelines1 state that a urine specimen with specific QUALITY CONTROL
gravity level of less than 1.003 is an indication of adulteration. For best results, performance of reagent strips should be Specific gravity and creatinine values should be considered confirmed by testing known negative and positive specimens or together to provide a better picture of whether the sample is controls whenever a new test is performed or whenever a new bottle is first opened. Each laboratory should establish its own goals for adequate standard of performance, and should Pyridium Chlorochromate: The presence of any chromate in
question handling and testing procedures if these standards are urine is indicative of adulteration as chromate is not a normal LIMITATIONS
Comparison to the color chart is dependent on the interpretation 1. U.S. Department of Transportation, Drug Testing of the individual. It is therefore, recommended that all laboratory personnel interpreting the results of these strips be 2. Young, al, Clinical Chemistry, 21 (9), 1975. tested for color blindness. As with all laboratory tests, 3. Friedman, R.B. Clinical Chemistry: 26 (4), 1980. definitive diagnostic or therapeutic decisions should not be 4. Tietz, N.W. Clinical Guide to Laboratory Tests, Third based on any single test result or method. Edition, W.B. Saunders Company, p566, 1995. 5. Chu, S.Y. and Sparks. Clinical Chemistry, 17, 1984. Some compounds or physical properties that may affect the test 6. Tingsrud, K.M. and Linne, J.J., Urinalysis and body Fluids result are listed below. Medications that discolor the urine may A color Test and Atlas, Mosby-Year Book, Inc., 1995. also cause abnormal results due to masking of the reactions of 7. Henry, J.B. et al.: Clinical Diagnosis and Management by Laboratory Methods, 16th Ed. Philadelphia: Saunders; EXPECTED VALUES
Creatinine: Daily creatinine excretion, related to muscle mass
of the human body, is usually constant6. The DOT guideline1 states that urine specimens with creatinine levels of less than 20 mg/dl are indications of adulteration. Although these ranges are affected by age, sex, diet, muscle mass and local population distribution2, sample with creatinine level of lower than 20 mg/dl should be considered adulterated.

Glutaraldehyde is not a natural component
of human urine and it should not be present in normal urine.
The presence of glutaraldehyde in the urine sample indicates



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