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Relative bioavailability of topiramate administered rectally Jeannine M. Conway , Angela K. Birnbaum , Robert L Kriel , James C. Cloyd a Experimental and Clinical Pharmacology, Epilepsy Research and Education Program, College of Pharmacy, University of Minnesota, 7-170 WDH 308 Harvard St. SE, Minneapolis, MN 55455, USA b Departments of Pediatrics and Neurology, Hennepin County Medical Center, Minneapolis, MN, USA Received 18 October 2002; received in revised form 12 March 2003; accepted 18 March 2003 Abstract
Objective: To determine the relative bioavailability and tolerability of a topiramate (TPM) suspension after rectal administra- Design/method: Seven healthy men and five healthy non-pregnant women were enrolled. A 100 or 200 mg tablet of TPM was given orally and a 200 mg dose was given rectally in a randomized, open-label, crossover study with at least a 2-week washoutperiod between doses. Plasma samples were collected prior to dosing and the following times after each dose: 0.5, 1, 1.5, 2, 3,4, 6, 8, 12, 16, 24, 48, 72, and 96 h. Relative bioavailability was determined by calculating the ratio of the dose-normalized areaunder the curve (AUC/D) for the rectal and oral doses.
Results: Ten subjects completed the study. Two of the first seven subjects who received a 200 mg initial oral dose, withdrew because of side effects. The remaining subjects received a 100 mg oral dose. Three subjects received a 200 mg dose orally andrectally, and seven subjects received 100 mg orally and 200 mg rectally. The average AUC/D was 0.72±0.18 h/l for the rectal doseand 0.76 ± 0.20 h/l for the oral dose. The relative bioavailability (n = 10) for TPM administered rectally was 0.95 ± 0.17 witha range of 0.68–1.2. There were no statistically significant differences between the oral or rectal pharmacokinetic parameters.
Conclusions: In healthy adults, rectally administered TPM is absorbed to a similar extent as the oral dosage form. Rectal administration is an acceptable route of administration for TPM, when the oral route is temporarily unavailable.
2003 Elsevier Science B.V. All rights reserved.
Keywords: Topiramate; Rectal administration; Bioavailability 1. Introduction
seizures (Although noncom-pliance is the most common reason for interruptions A common cause of seizure breakthrough is of oral therapy, there are a variety of other reasons interruption in antiepileptic drug (AED) therapy such as vomiting, gastrointestinal illness, fasting prior to and after surgery, and impairment of conscious- catastrophic effects on a patient’s seizure control, in- ness that may result in a precipitous decrease in AED cluding increased frequency, intensity, or duration of Alternatives to oral AED therapy are limited.
Among all the AEDs only three are available as Corresponding author. Tel.: +1-612-625-2999; parenteral formulations: phenobarbital, phenytoin, E-mail address: [email protected] (J.M. Conway).
and sodium valproate. This leaves many patients 0920-1211/03/$ – see front matter 2003 Elsevier Science B.V. All rights reserved.
doi:10.1016/S0920-1211(03)00083-4 J.M. Conway et al. / Epilepsy Research 54 (2003) 91–96 and clinicians with the dilemma of using a different suspension in a volume ≤20 ml. TPM is also lipophilic AED based on availability of a parenteral solution.
enough (log P = 0.573) to facilitate relatively rapid Such circumstances expose the patient to both unpre- dictable side effects and the potential loss of seizure control. Even when a parenteral formulation is avail- The objective of this study was to determine the able, administration requires the presence of skilled relative bioavailability and tolerability of TPM sus- medical personnel in a healthcare facility with all pension after rectal administration using a formulation the attendant costs and inconveniences. The preferred that is easily prepared from commercially available approach would be to maintain the patient on her or tablets. Information on the absorption and safety of his current medication by using an alternate route of rectal administration of TPM can guide the practi- tioner when oral administration is not possible.
Rectal administration may be used as a bridge when oral AED therapy is not possible and a parenteralformulation does not exist or is impractical. An ac- 2. Methods
ceptable alternative to the oral route may be rectaladministration if the drug is adequately and consis- tently absorbed, and the formulation is well tolerated.
Many AEDs possess physical–chemical characteris- The subject population was healthy volunteers from tics that make them candidates for rectal administra- 18 to 65 years of age. Subjects were excluded from the study if they had a previous history of nephrolithiasis, were not in good health, were taking other medica- were unwilling or unable to receive medications rectally, were unwilling or unable to tolerate multi- ple venipunctures, or had a hemoglobin <12 g/dl. The study was approved by the IRB’s at both the Univer- These studies demonstrate that it is possible to take sity of Minnesota (Minneapolis, MN) and Hennepin commercially available oral formulations and, with a County Medical Center (Minneapolis, MN). Subjects few simple steps that can be done in the pharmacy or were informed of the study and gave written consent.
For each phase of the study, subjects were admitted Topiramate (TPM) is an AED approved for use to the DaVita Clinical Research Unit (CRU) located as adjunctive therapy for partial onset seizures or at Hennepin County Medical Center, where they re- primary generalized tonic clonic seizures in children mained for approximately 24 h. A 100 or 200 mg (ages 2–16) years and adults. It is also indicated as tablet of TPM was given orally or a 200 mg dose was adjunctive therapy for children 2 years of age or older given rectally in a randomized, open-label, crossover with seizures due to Lennox–Gastaut syndrome. TPM study with at least a 2-week washout period between is available only as oral formulations: 25, 100, and doses. The original study design was for each subject 200 mg tablets or 15 and 25 mg sprinkle capsules. It to receive a 200 mg oral dose; however, two of the has multiple mechanisms of action that work for many subjects that received a 200 mg oral dose withdrew from the study due to adverse events. Hence, the suggests, it is useful for childhood epilepsies including remaining subjects received a 100 mg oral dose. For the oral dose, the subject swallowed either the com- mercially available 100 or 200 mg tablet with 120 ml of water. The 200 mg dose was selected based on the predicted concentrations that would be seen in TPM would appear to be a viable candidate humans following a single dose and the sensitivity of for rectal administration. Its chemical–physical prop- erties indicate that it is sufficiently soluble in water The rectal dose was prepared by crushing a 200 mg (9.8 mg/ml) to deliver a clinically relevant dose as a tablet with a mortar and pestle, and adding 10 ml of J.M. Conway et al. / Epilepsy Research 54 (2003) 91–96 tap water to suspend the material. The contents of the gation. The eluate was evaporated to dryness under mixture were then drawn into a 35 ml syringe and the nitrogen and the final samples were reconstituted with mortar was rinsed with 5 ml of water twice and then 100 ␮l of toluene. A 2 ␮l sample was injected on to drawn into the syringe after each rinse. The syringe the column and quantified by GC-MS in the SIM was inverted 10 times to mix the contents. The sub- mode. The GC-MS system consisted of a HP5890 jects were placed in the right lateral decubitus position Series II Gas Chromatograph; HP5971 Mass Selec- and the 35 ml syringe with an attached catheter was in- tive Detector; HP7673 Autoinjector; using a DB-5MS serted approximately 2.5 in. into the rectum. The sub- 30 m ×0.25 mm i.d. capillary of 0.5 ␮m film thickness jects rested in a supine position for at least 60 min and column (J&W Scientific, Folsom, CA). The program were monitored to ensure that rectal expulsion did not for sample analysis was an injection volume of 2 ␮l, transfer line temperature of 280 ◦C, initial tempera- Plasma samples were collected just prior to and ture of 230 ◦C, initial time of 0.5 min, program rate at the following times after each dose: 0.5, 1, 1.5, 2, was 10 ◦C/min, final temperature of 290 ◦C, final time 3, 4, 6, 8, 12, 16, 24, 48, 72, and 96 h. Blood sam- of 1.0 min, and detector temperature of 300 ◦C. Ions ples were centrifuged and plasma was transferred to monitored were 193.1 and 324.1 mz. The peak times a separate container for storage at −80 ◦C. Adverse were 4.5 min (TPM) and 5.4 min (I.S.).
event data were collected, as a self-report, at each Each subject’s oral and rectal blood samples were blood sample collection. The scale was designed to extracted and analyzed on the same day and compared measure the level of comfort of the side effects that on the same standard curve. Triplicate quality con- were thought to be the most likely to occur: speech trol samples were run with plasma samples and were impairment, dizziness, nausea, headache, difficulties deemed acceptable if the values were within two stan- in concentrating, sleepiness, unusual sensations, dis- dard deviations of the mean quality control values for comfort due to administration of the rectal dose, and all previous runs. The solubility of the suspension was abnormal vision. Severity of adverse events was rated determined by preparing the rectal dose, as described on a scale from 0 to 5, with 0 indicating no notice- above, and filtering the suspension. Unextracted trip- able adverse event and 5, a severe adverse event. Side licate samples and standard curves were analyzed by effects and discomfort associated with oral and rec- tal administration were assessed. Subjects were alsoasked to report adverse events not specifically listed.
4. Pharmacokinetic analyses
3. Sample analysis
All pharmacokinetic analyses were completed with WinNonlin version 3.1 (Pharsight, Mountain View, TPM concentrations were determined by a GC-MS CA) using a noncompartmental approach. The area method that was modified from one described by under the TPM plasma concentration time curve was determined using the trapezoidal rule from time laboratory. TPM reference standard was provided by 0 to 96 h (AUC0–96). The total AUC was calcu- Johnson & Johnson Pharmaceutical Research and lated by adding the AUC96–∞ (C96/kel) to AUC0–96.
Development (Raritan, NJ). For extraction, 50 ␮l of The elimination rate constant (kel) was determined the internal standard, cyheptamide (Sigma, St. Louis, by the log-linear regression analysis of the termi- MO) and 500 ␮l of saturated ammonium acetate were nal plasma TPM concentration time points. Relative added to 500 ␮l of sample or standard. Samples were bioavailability (Frel) was determined by calculating vortexed and transferred to an EmporeTM (3M, St.
the ratio of the dose-normalized area under the con- Paul, MN) C18 solid-phase extraction disk cartridge.
centration time curves (AUC0–∞/D) for the rectal The disk was primed and the sample was forced and oral doses using the following equation: Frel = through the disk by centrifugation. The disk was then (AUC0–∞,rectal/Drectal)/(AUC0–∞,oral/Doral). We as- washed with water, hexane, and finally ethyl acetate sumed linear pharmacokinetics for both the 100 and was added and forced through the disk by centrifu- J.M. Conway et al. / Epilepsy Research 54 (2003) 91–96 5. Statistical analysis
The mean and standard deviation were determined for all pharmacokinetic parameters. ANOVA was usedto compare the oral to the rectal treatments (MircosoftExcel 2000). A P ≤ 0.05 was considered statisticallydifferent. Bioequivalence was determined using Win-Nonlin version 3.1 and the Westlake method.
6. Results
Fig. 1. Relative bioavailability of each subject. Each diamondrepresents an individual subject.
Ten of 12 subjects, who gave consent, completed the study (Two of the first seven subjectswithdrew because of side effects following the ini-tial 200 mg oral dose. Because of the unacceptableadverse effects, the oral dose was reduced to 100 mgfor the remaining subjects. Three subjects received200 mg orally and rectally and seven subjects received100 mg orally and 200 mg rectally. Our laboratory de-termined the solubility of the rectal TPM formulationmade from the 200 mg tablet to be 2.78 mg/ml.
The average AUC0–∞/D for the rectal and oral doses was 0.72 ± 0.18 h/l and 0.76 ± 0.20 h/l, respec-tively There were no statistical differencesbetween the pharmacokinetic parameters for the oral Fig. 2. Average plasma concentrations (dose adjusted) vs. time.
and rectal doses. The relative bioavailability (n = 10) Error bars signify one standard deviation of the mean.
for TPM administered rectally was 0.95 ± 0.17 with a Subject characteristics for pharmacokinetic analysis (n = 10) range of 0.68–1.2 The average dose adjusted AUC0–∞ for 10 subjects is shown in Rectal TPM bioavailability for 9 of 10 subjects fell within a range of 80–120%. The 90% confidence interval bounds for the least-squares mean ratio (rectal/oral) from log transformed data was 81.9–118.
a Values divided by dose for statistical comparison.
J.M. Conway et al. / Epilepsy Research 54 (2003) 91–96 Table 3Side effects vs. route: a comparison of the highest score reported by a subject at any time point the time it occurred Side effects were rated from 0 (no discomfort) to 5 (severe discomfort).
The subjects that completed the study reported no neither difference was statistically different. There impairment of speech or discomfort due to adminis- were no statistical differences between the routes tration by either route. Dizziness, nausea, headache, of administration or other pharmacokinetic param- difficulties in concentrating, sleepiness, unusual sen- eters including clearance, half-life, elimination rate sations, and abnormal vision occurred regardless of constant, or volume of distribution.
the route of dose delivery. If side effects were re- The variability in rectal TPM bioavailability could ported, they generally occurred between 1 and 4 h be due to several factors. We found that the amount following the dose, and resolved by 8 h regardless of of a crushed 200 mg tablet that dissolves in water is the route of medication. The two subjects (#4 and #7), 2.78 mg/ml. This is much lower than the predicted who discontinued the study following the oral dose, solubility of 9.8 mg/ml. Despite the solubility being reported adverse events. The highest score subject #4 less than expected, it appears that an adequate amount reported for any given side effect was 1, and the sub- was absorbed rectally. Drug dissolution can be af- ject withdrew following difficulty with motor skills fected by the fineness of the particles, the presence and cognitive function after the oral dose. Subject #7 of other excipient particulates, and water temperature reported numerous side effects with a score up to 5 and withdrew from the study after reporting “trouble of these factors varied when preparing each subject’s in concentrating” during the entire week after the rectal dose. Rectal doses with a high percentage of oral dose. lists the medians and modes of TPM in suspension, as opposed to solution, may have the highest value, for the adverse events listed above, resulted in lower bioavailability. The assumption of reported by the subjects at any given time point.
linear pharmacokinetics between the 100 and 200 mgdoses was made for the bioavailability calculation. Ifthe pharmacokinetics of TPM is not linear between 7. Discussion
100 and 200 mg doses, the calculations may under-estimate or overestimate bioavailability. Nonetheless, In healthy adults, rectally administered TPM sus- rectal TPM displays high and relatively consistent ab- pension is bioequivalent to the orally administered sorption relative to other rectally administered AEDS tablet. This study demonstrated a relative bioavailabil- ity of 0.95 ± 0.17 for rectally administered TPM with a 90% confidence interval of 81.9–118. The plasma Our data suggest that a rectally administered TPM concentrations attained by rectal administration 10 mg/ml suspension of a crushed TPM tablet may tended to be lower and peak plasma concentration be used when oral administration is not possible. Vol- tended to occur later compared to the oral dose, but ume considerations may limit the usefulness of this J.M. Conway et al. / Epilepsy Research 54 (2003) 91–96 formulation if patients require dosages greater than Cloyd, J.C., Kriel, R.L., 1981. Bioavailability of rectally 200 mg per dose. Alternatively, given the low solubil- administered valproic acid syrup. Neurology 31 (10), 1348– ity of the rectal formulation used in this study, it may Cloyd, J.C., Lalonde, R.L., et al., 1998. A single-blind, crossover be possible to achieve the same results with a more comparison of the pharmacokinetics and cognitive effects of a concentrated suspension. The plasma concentrations new diazepam rectal gel with intravenous diazepam. Epilepsia attained following rectal administration should be sufficient when replacing a dose if a person cannot Dhillon, S., Oxley, J., et al., 1982. Bioavailability of diazepam after take their regular dose orally. The T intravenous, oral and rectal administration in adult epileptic patients. Br. J. Clin. Pharmacol. 13 (3), 427–432.
tion half-life indicate that rectal TPM can be given at the same interval as oral TPM (once or twice daily).
pharmacokinetics and effect of food on the bioavailability This information will help guide the clinician and of topiramate, a novel antiepileptic drug. J. Clin. Pharmacol.
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be useful in situations where patients on TPM go Graves, N.M., Kriel, R.L., et al., 1985. Relative bioavailability into status epilepticus due to an abrupt decrease in of rectally administered carbamazepine suspension in humans.
plasma concentrations (e.g. noncompliance, malab- sorption, drug interactions, etc.). Until further in- Graves, N.M., Holmes, G.B., et al., 1989. Relative bioavailability of formation is available, dose substitution should be rectally administered phenobarbital sodium parenteral solution.
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