Microsoft word - effect_of_pentoxifylline_added_to_frozen[1].doc
EFFECT OF PENTOXIFYLLINE ADDED TO FROZEN-THAWED SEMEN ON SHEEP FERTILITY
D. Fernández Abella 1,2, C. Bonilla Riera 2, O. Irabuena 3 and S. Sterla 3
1 Secretariado Uruguayo de la Lana. Dpto de Producción Ovina. Rbla. B.Brum 3764. 11800 Montevideo,Uruguay
2 Facultad de Agronomía, Universidad de la República. R. N. 50000. Salto, Uruguay
3 Laboratorio de Inmunología. Universidad de la República. R.N. 50000. Salto, Uruguay
INTRODUCTION
In our country, the sheep fertility obtained by
linear-array transrectal probe). Lambing was
intrauterine artificial insemination using frozen-
determined by udder examination 150 days after
thawed semen has been very inconsistent (30-60%).
insemination and verified by the number of lambs
In several especies (human, dog, equine, pig)
pentoxifylline (PTX) has been used as an additive
to sperm to improve sperm characteristics. The
RESULTS AND DISCUSSION
mechanism of action on spermatozoa is generally
No effect of GnRH administration on the pregnancy
rate and lambing was detected (fertility 46.6 vs.
phosphodiesterase activity, resulting in elevation of
48.3; fecundity 56.6 vs. 54.2 %, T1 and T2
complementary adenosine monophosphate levels in
respectively). When PTX was used , the volume of
spermatozoa. PTX enhanced post-thaw motility and
the dose affected the lambing rate (46.3 vs. 62.3,
membrane integrity when added after thawing (1).
P<0.10, A vs. B). The PTX at 0.25 mL of dose (B)
ON the other hand, GnRH administration at 35
had a tendency to improve the lambing rate (62.3
hours after pessary removal improves pregnancy
vs. 51.8, B and C, respectively). There was an
rates in progestagen-treaded ewes (2). GnRH
interaction between PTX addition and GnRH
induces ovulation in all the ewes. The aims of this
administration (P<0.05) which determined the
study were to compare the effects of pentoxifylline
highest fertility and fecundity (41.5 vs 58.0; 53.7
vs. 73.7%, T1B vs. T2B fertility and fecundity,
respectively). A significant improvement in
fertilization rate was also demonstrated in human
MATERIALS AND METHODS
and other animal species. The improvement of
Two hundred and forty multiparous Australian
sperm characteristics by PTX is most important
Merino ewes of 4 to 6 years old were separated at
when the female fertility is better (T2). In our case
random in two groups: (T1) Progestagen-
the fecundity increased because both male (PTX
impregnated intravaginal pessaries containing 60
added to sperm) and female fertility (GnRH
mg of medroxy progesterone acetate were inserted
administration) were enhanced. These results
for 14 days to synchronize estrus. At pessary
indicate that pentoxifylline associate to GnRH
removal eCG (350 UI.) was administered; and (T2)
administration 35 hours after pessary removal
the same treatment plus 10 µg GnRH administration
at 35 hours after pessary removal. These treatments
were later broken into three subgroups in order to
REFERENCES
add PTX to the thawed semen fifteen minutes
(1) Stanic, P. Sonicki, Z. &Suchanek, E. (2002). Int.
before intrauterine insemination: These subgroups
were: A) the addition of 2.5 mM PTX in 0.4 mL
(2) Fernández Abella, D. & Villegas, N. (2002).
dose volume, B) the addition of 2.5 mM PTX in
0.25 mL dose volume, C) control without PTX and
0.25 mL of dose volume, containing the same
number of spermatozoa (40 x 106). Thus, the
experimental design was a 2 x 3 factorial. In a
concentrations were evaluated (2.0, 2.5, 3.5, 5.0, 7.0
mM). The most beneficial effect (motility) was
observed when 2.5 mM of PTX was added to
semen in the process of thawing. Pregnancy was
diagnosed and fetuses counted by ultrasonography
30 days after insemination (Aloka SSD 500; 5MHz,
SYNCHRONIZE + 10 µg GnRH TREATMENT FERTILITY 46,6 FECUNDITY 56,6 54,2 PENTOXIFYLLINE PENTOXIFYLLINE PENTOXIFYLLINE CONTROL A.I. DOSE VOLUME A.I. DOSE VOLUME ( thawed semen) FERTILITY 47,5 FECUNDITY P<0.10 PENTOXIFYLLINE 350 PMSG A.I. DOSE VOLUME 0.4 + 10 µg GnRH FERTILITY 41,5 FECUNDITY 53,7 73,7 P<0.05
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