Npc10.ebc.ee

Mechanism of photoinhibition: magnetic field effect, singlet oxygen and
kinetics

Esa Tyystjärvi1, Marja Hakala-Yatkin1, Päivi Sarvikas1, Heta Mattila1, Sirin Dönmez1, Taina Tyystjärvi1, Petriina Paturi2, Ladislav Nedbal3 1Molecular Plant Biology, Department of Biochemistry and Food Chemistry, FI-20014 University of Turku, Finland; 2Department of Physics, FI-20014 University of Turku, Finland; 3Department of Biological Dynamics, Institute of Systems Biology and Ecology CAS, Zamek 136, 37333 Nove Hrady, Czech Republic Magnetic fields in the range of 100 mT are known to accelerate plant growth, but the phenomenon has lacked an explanation. We tested the hypothesis that magnetic fields lower the triplet yield of the recombination of the primary radical pair and thereby limit production of singlet oxygen (1O2). We found that magnetic fields really offer some protection against loss of PSII activity in high light, confirming that charge recombination mediates the formation of 1O2. However, the magnetic field effect disappeared in vitro and also in the presence of lincomycin in vivo, indicating that 1O2 exerts its effect on PSII by inhibiting PSII repair cycle, not by causing direct damage to PSII. Triplet chlorophyll is also produced by intersystem crossing which is insensitive to moderate magnetic fields. To measure the importance of 1O2 produced by types of chlorophyll triplets, we illuminated leaves of the Arabidopsis vte1 mutant lacking α-tocopherol, an important 1O2 scavenger. Leaves of the vte1 mutant were found to be more susceptible to photoinhibition than the wild type in the absence but not in the presence of lincomycin. The result confirms the finding that 1O2 is harmful to PSII mainly because 1O2 inhibits repair of photoinhibited PSII. Short flashes have been suggested to cause photoinhibition because S recombination produces triplet chlorophyll. We tested the photoinhibitory efficiency of saturating single-turnover Xenon flashes in vivo and in vitro and found that the photoinhibitory efficiency of Xenon flashes is directly proportional to flash energy and independent of the time interval between the flashes. Because a saturating flash always causes the same number of recombination reactions irrespective of flash energy, the result indicates that 1O 2 produced due to S2QB recombination does not harm PSII. In fact, the quantum yield of photoinhibition was the same, whether Xenon flashes or continuous light was used, suggesting that flashes cause photoinhibition with the same mechanism as continuous light. Thus, photoinhibition is largely independent of PSII electron transport, which supports our earlier suggestion that the manganese ions of PSII have a role in photoinhibition. Experiments with the npq4 and npq1 mutants of Arabidopsis demonstrated that non-photochemical quenching (NPQ) lowers the rate constant of photoinhibition by 25 %. We also tested whether the qI type of NPQ, associated with photoinhibited PSII centres, could protect the remaining active centres. Such protection would make the rate constant of photoinhibition to decrease with proceeding photoinhibition. However, photoinhibition did not deviate from first-order kinetics when leaves were illuminated for several hours in the presence of lincomycin. Also in vitro, photoinhibition strictly followed first-order kinetics until oxygen evolution was completely lost. Thus, qI does not offer any protection in addition to qE. Furthermore, persistent first-order kinetics indicates that the protective effect of NPQ must act on a separate, chlorophyll-dependent photoinhibition mechanism rather than acting on a second step of a manganese mechanism.

Source: http://npc10.ebc.ee/abstracts/Esa_Tyystjarvi.pdf

Microsoft word - bag o medsbest.doc

Deborah J McCoy-Freeman, BS, RN, NREMT-P Objectives • Discuss a variety of commonly prescribed medications • Discover the relationship between certain medications and their indication leading to more information of the past • Describe interviewing techniques for achieving better assessment results 70 yo female, C/O dizziness and light headedness. Poor historian. S: dizziness, confusi

Microsoft word - toxic plant list - dogs.doc

Plants Toxic to Dogs Adam-and-Eve (Arum, Lord-and-Ladies, Wake Robin, Starch Root, Bobbins, Cuckoo Plant) | Scientific Name: Arum maculatum | Family: Araceae African Wonder Tree | Scientific Name: Ricinus communis | Family: Alocasia (Elephant's Ear) | Scientific Name: Alocasia spp. | Family: Araceae Aloe | Scientific Name: Aloe vera | Family: Liliaceae Ama

Copyright ©2010-2018 Medical Science