Whatman Protein Precipitation UNIFILTER® Fast Flow
Fast, easy and automatable protein precipitation.
A fast, effective protein removal method for plasma
PROTOCOL
and serum is vital for high-throughput labs measuring
drugs and metabolites. Whatman provides the ideal
solution with the Fast Flow Protein Precipitation
UNIFILTER®. This high-quality filterplate replaces
the lengthy centrifugation process with a vacuum
filtration method, making sample preparation three
times faster. It allows you to automate acetonitrile
precipitation and speed your research. Superior plate
construction ensures no well-to-well crosstalk, while
two specially formulated filters prevent filter clogging
plasma (≤ 150 µL)or serum (≤ 200 µL)
Features and Benefits
is three times faster than centrifugation.
• Easy-to-use Filterplate is automatable,
less labor-intensive and highly productive
• Minimal liquid handling Precipitation
and filtration are performed in one well. Applications EXAMPLES AND RESULTS
Samples (100 µL) of serum and plasma were
Precipitation plate and a conventional spin
98.8% 99.2% 97.5% 98.9% otein Removal
clarification. The centrifugation method was
carried out by acetonitrile precipitation of
plasma serum
sample in microcentrifuge tubes followed
Sample Type
aspiration of clarified sample. All samples
Whatman is a global leader in separations technology
saline solution and analyzed for protein
and is known in the scientific community for providing
content using the micro-BCA assay (Pierce).
innovative Life Science products and solutions. Our instinct
for simplification accelerates the rate of discovery, reduces
costs and saves time. In order to focus on the unique needsof customers, Whatman is organized into four business
protein with high efficiency (~98%).
development units: LabSciences, BioScience, MedTech-Diagnostics and MedTech-Devices. For more information, visit www.whatman.com.
Whatman and UNIFILTER are registered trademarks, and
UNIPLATE, UniVac and VacAssist are trademarks of the
y = 1.096x - 26.841 R2 = 0.973 feine Conc.
(λmax 275 nm) overlaps the protein range
(280 nm) so accurate quantitation requires
efficient protein removal. Various concen-
Observed Caf
trations of caffeine were added to plasma
Expected Caffeine Conc.
vs. expected caffeine concentrations, with
an excellent correlation (R2 = 0.973).
Tel: + 44 (0)1622 676670Fax: + 44 (0)1622 691425E-mail: [email protected]
Forty-eight aliquots of plasma containing
Precipitation UNIFILTER. The filtrates were
otein Reduction
(Pierce) and for caffeine by uv absorbance.
Asia Pacific Whatman Asia Pacific Pte Ltd
The results show consistently high protein
Singapore 169877Tel: +65 6534 0138Fax: +65 6534 2166E-mail: [email protected]WHATMAN CATALOG ORDERING INFORMATION Catalog Number Description Qty/Case 7720-7236 Protein Precipitation UNIFILTER Fast Flow 7701-5750 UNIPLATE™—PP, Round Well, Round Bottom, 750 µL/96 well 7701-5200 UNIPLATE—PP, Square Well, Round Bottom, 2000 µL/96 well 7705-0102 UniVac™ 3—Anodized Aluminum 7705-0107 UniVac 3—Acrylic Leaders in Separations Technology 7705-0112 VacAssist™ Vacuum Assist Frame—Teflon® www.whatman.com 7705-0205 Teflon/Silicone Sealing Gasket
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Whatman Protein
EXAMPLES AND RESULTS