Microsoft word - clinical laboratory sciences.doc

DEPARTMENT OF CLINICAL LABORATORY SCIENCES
Research Report 2004
Division of Chemical Pathology

(Including the Chemical Pathology sections of Groote Schuur Hospital and Red Cross War Memorial
Children’s Hospital)
Head of Division: Associate Professor H.E. Henderson (Acting Head)
Divisional Profile

The Division of Chemical Pathology and the NHLS offers a comprehensive dedicated routine
service to both the adult and paediatric patients attending the Groote Schuur and Red Cross
Children’s Hospitals. It participates actively at the laboratory/ward interface by attendance
at ward rounds and clinical meetings. There is a strong specialist diagnostics and research
focus on metabolic disorders of acquired and familial aetiology. Teaching commitments
address both undergraduate and postgraduate needs, with several MSc, PhD and MMed
students under current supervision. Research activities cover the biochemical mechanisms
of ion and drug pumps, the identification of new drug targets in Tuberculosis and
Leishmaniasis, the molecular genetics of inherited metabolic disorders, mitochondrial
myopathies and erythrocyte antioxidant biochemistry.
Divisional Statistics
Permanent and Long-term Contract Staff

Associate Professors
Total 20

Students
Doctoral
Undergraduate 159
Total 170

Research Fields and Staff
Permanent Staff

A/Professor Howard E. Henderson
Chief Specialist Scientist; biochemical diagnosis of diseases of sick children; genetics of
hyperlipidaemia; tuberculosis; galactosaemia; and cystic fibrosis; inherited metabolic
disease; primary immunodeficiencies; post graduate teaching
A/Professor David B. McIntosh
Chief Specialist Scientist; ion pumps and ABC transporters; P-type ATPase; calcium-
ATPase; multidrug resistance P-glycoprotein; flavonoids; site-directed mutagenesis;
photoaffinity labelling; malaria; post graduate teaching
A/Professor Daan J. Steenkamp
biochemical parasitology, enzymes in the metabolism of the trypanosomatids and
mycobacteria as potential drug targets, thiol metabolism in pathogens; post graduate
teaching
Dr Peter A. Berman
Principal Specialist; carbohydrate-deficient transferrin as a biochemical marker of
alcoholism, red cell chemistry and biochemistry, urate metabolism, metabolic disorders,
undergraduate and post graduate teaching
Dr Heleen Vreede
Senior Specialist; oversees routine laboratory service at GSH; method development;
information technology; post graduate teaching
Dr Wessel Meyer
Specialist; oversees routine laboratory service at RXH; calcitonin and CRP in paediatric
practice; snake bite; undergraduate and post graduate teaching
Dr Judy A. King
Principal Specialist Scientist; hypertension; renin; aldosterone; sodium channel mutations;
routine diagnostic reviewing; post graduate teaching
Dr E. Patricia Owen
Principal Specialist Scientist; inherited metabolic disease; homocysteine; mitochondrial DNA
mutations; TPMT mutations; urea cycle disorders; hypertension; preelampsia, epithelia
sodium channel mutations; post graduate teaching
Ms Felicity Leisegang
Medical Scientist; molecular genetics of galactosaemia; spinal muscular atrophy; cystic
fibrosis; application of PCR to genetic screening; post graduate teaching
Honorary Professors

Emeritus Professor Eric Harley
purine and pyrimidine metabolism; comparative biology of red cell antioxidant systems
Divisional Contact Details
Postal Address: Division of Chemical Pathology, University of Cape Town Medical School,
Observatory, 7925
Telephone: (SA) (21) 406 6185
Fax: (SA) (21) 448 8150
E-mail: [email protected]
CLINICAL LABORATORY SCIENCES
DIVISION OF FORENSIC MEDICINE AND TOXICOLOGY

(Including the Gender, Health and Justice Research Unit)
Head of Division: Professor L.J. Martin
Divisional Profile

A range of activities related to the role of Forensic Medicine in public policy and health
promotion are being pursued. These relate specifically to violence against women and
children, the role of drugs and toxins in deaths, and firearm injuries. The provision of a
sustainable database on violence and injury in the Cape Town Metropole is being
implemented as an urgent research priority.

Divisional Statistics

Permanent and Long-term Contract Staff

Chief Specialist/Professor
Specialists 1 Registrars 2 Principal Medical Officer (part-time) Total 9

Students

Undergraduate fourth year
Final year LLB students (Medicina Forensis course) Total 190

Research Fields and Staff

Permanent Staff

Professor L.J. Martin
management protocols for rape survivors; domestic homicide; monitoring implementation of
rape law reforms; domestic violence guidelines for the health sector; epidemiology of female
murder
Dr D. Lourens
undetermined causes of death; deaths related to traditional medicine; drug related death

Dr Y.Y. van der Heyde
the effects of pre-natal alcohol exposure; SIDS; paediatric mortality
firearm fatalities and final judicial outcome

Divisional Contact Details

Postal Address: Division Forensic Medicine and Toxicology, Faculty of Health Sciences,
Anzio Road, Observatory, 7925
Telephone: SA (21) 406 6412
Fax: SA (21) 448 1249
E-mail: [email protected]
GENDER, HEALTH AND JUSTICE RESEARCH UNIT

Director: Lillian Artz
Executive Director of Unit: Professor Lynette Denny (Department of Obstetrics and
Gynaecology)
Unit Profile

The mission of this Unit is to improve service provision to victims of violence against women
in South Africa through research, advocacy and education. Drawing together established
researchers with a strong record of social-action research in disciplines including law,
criminology, forensic sciences and pathology, gynecology, and psychology, this Unit is the
first of its kind in South Africa to respond to the pressing need for research and interventions
that crosses disciplines in support of our efforts to address the alarmingly high levels of
violence against women in this country. The Unit fulfils its mission by conducting rigorous
evidence-based research into:
ƒ South African women’s experiences of sexual and gender-based violence;
ƒ The responses of women, their communities and the criminal justice, health, and welfare
ƒ The intersections and disjunctures between health and criminal justice responses to ƒ The developmental context in which violence occurs, and the role of violence in perpetuating women’s under-development; ƒ The implementation difficulties of translating ‘law on paper’ into ‘law in practice’ in health, criminal justice and medico-legal settings; ƒ The development of indicators for monitoring the implementation of progressive legislation and compliance by criminal justice and health sector personnel with statutory duties; ƒ International and local models of best practice for addressing violence against women.
Unit Staff
Research Staff

Research Fields and Staff

Lillian Artz
criminology; feminist legal theory; women and law reform; sexual offences; domestic
violence; commercial sex work; research ethics; HIV
Dee Smythe
rule of law; violence against women; human rights; women and law reform; development
Kelley Moult
forensic criminology; domestic violence; informal justice
Katie Doolan
crime and violence prevention; violence against women; HIV
Ayesha Sasman
sexual offences; HIV and post-exposure prophylaxis; mental health consequences of rape
and HIV
Nomthandazo Kate Makeleni
sexual offences; HIV and post-exposure prophylaxis; mental health consequences of rape
and HIV
Fazielah Bartlett
sexual offences; HIV and post-exposure prophylaxis; mental health consequences of rape
and HIV
Tandie Jostina Fipaza
sexual offences; HIV and post-exposure prophylaxis

Unit Contact Details

Postal Address: H52 Old Main Building, Groote Schuur Hospital, Observatory, 7935
Telephone: SA (21) 406 6022/3
Fax: SA (21) 406 6020
E-mail: [email protected] or [email protected]
Webpage: http://www.uct.ac.za/generic.php?m=/departments/genhealthjust/index.php
CLINICAL LABORATORY SCIENCES
DIVISION OF HAEMATOLOGY
Head of Division: Professor N. Novitzky
Divisional Profile
The Division of Haematology at Groote Schuur Hospital is a complex unit with
multidisciplinary activities that include outpatient clinics, admission beds as well as
diagnostic and research laboratories. The clinical services are organised under the umbrella
of the Department of Internal Medicine, while the diagnostic sections are under the broader
organisation of the Department of Laboratory Medicine and NHLS. Patients with elaborate
diagnostic problems, bone marrow failure or requiring stem cell transplantation are referred
from all provinces; particularly the Northern and Eastern Cape and occasional y we receive
referrals from foreign institutions in Sub-Saharan Africa.
More recently this Division has undergone a significant transformation this year. The routine
section of the laboratories that is under the control of the National Health Laboratory Service
has now been rationalized and relocated into the C-floor of the hospital providing a multi-
disciplinary centralized laboratory service. This physical separation from the clinical section
is likely to substantially challenge the close physical and interpersonal interaction between
clinical and laboratory professionals, which for many years has maintained a unified
discipline. The service laboratories have been under considerable strain due to staff
shortages. In the academic section, this year again, no equipment budget was available to
replace critical diagnostic instruments.
On a more positive note, funding has now been made available for the creation of a scientist
post under the university establishment to expand our strong academic roots. The task of
this scientist will be to pursue with diligence further research into the area of haematopoietic
stem cell biology both at the basic sciences level in the area of stem cell transplantation and
into the various forms of application to improve the understanding and treatment for
haematological malignancies.

Divisional Statistics
Permanent and Long-term Contract Staff
UCT

Students
Doctoral 4
Masters 8
Honours 1
Postdoctoral 1
Undergraduate 375
Total 389

Research Fields and Staff
Permanent Staff
Professor N. Novitzky
haematopoietic stem cell biology; clonogenic assays; haematopoietic stem cell
transplantation; immune reconstitution; haematological malignancies
Dr Cecile du Toit
haematopoietic stem cell transplantation; haematological malignancies; haemophilia
Dr Jill Finlayson
molecular biology of neoplasia: chronic myelogenous leukaemia; coagulation abnormalities
Dr Gideon Hanekom
molecular detection of minimal residual disease in patients with solid tumours (in specific
breast cancer and melanoma) leukemias, lymphomas and patients with myelodysplastic
syndromes; immunotherapy of such cancers; molecular pathogenesis of these cancers
Dr Andrew McDonald
haemophilia; thrombophilia; stem cell transplantation

Contract Staff
Ms Rygana Mohamed
cellular biology of neoplasia; clonogenic assays

Divisional Contact Details
Postal Address: Division of Haematology, Room 6.06, Chris Barnard Building, Anzio Road,
Observatory, 7925
Telephone: SA (21) 406 6154 or 404 3073
Fax: SA (21) 448 8607
E-mail: [email protected]
CLINICAL LABORATORY SCIENCES
DIVISION OF HUMAN GENETICS

(Including the MRC/UCT Human Genetics Research Unit and the CANSA Colorectal Cancer
Research Consortium)
Head of Division: Professor Raj S. Ramesar
Divisional Profile
The Division, which remains one of only two HPCSA-recognised training centres nationally,
has strengthened its clinical base with two senior registrars successfully completing the
Medical Genetics examinations within the College of Medicine. In recognition of international
trends towards a high quality service incorporating genetic counsel ing for individuals with
genetic/inherited disorders, an HPCSA-accredited MSc (Genetic Counselling) programme
was established in 2003.
A major aspect of the Division’s research agenda is to the identification of genes underlying
disease in South African populations. These findings are meant to inform patient care and
management. The Genomic Platform, for high-throughput genetic analysis involving
genotyping, mutation detection and DNA sequencing, is now operational and has increased
the workload within the Unit while offering spare capacity to other researchers in the Faculty
of Health Sciences at UCT and the Western Cape, generally.
The numbers of registered postgraduate students is unprecedented (overall this includes up
to 6 BSc (Med) Hons, 5 MSc and 5 PhD students). A supportive environment has led to five
postdoctoral researchers being accommodated and developed within the research facility.
Apart from teaching in the medical undergraduate programme, courses are provided within
the following programmes: BSc (Med) Hons, Medical Genetics subspecialist training and
MSc(Genetic Counselling). In addition, the Division has an active academic programme
which is comprised of CPD-accredited weekly journal clubs (Monday mornings), seminars
(Tuesday midday) and clinical ‘grand rounds’ (Tuesday afternoon). Furthermore,
researchers from other departments and other universities have been attracted to seek
accommodation and to conduct their research in the Division and take advantage of the
Human Genetics expertise and technology therein.
The ongoing Retinal Disorders research project has resulted in a large and invaluable
genetic registry. The most successful aspect of this project has been the recent identification
of the gene underlying a dominant form of retinitis pigmentosa, which we first mapped to
chromosome 17 in 1995 (Rebello et al., 2004). Research on the South African mutation, in a
collaborators lab, showed promise for a novel therapeutic intervention in individuals know to
be carrying the mutation. A clinical trial is currently under review. Following the
identification of genetic defects underlying disease, internationally-vetted protocols have
been developed for delivering diagnostic and predictive genetic testing for this group of
genetic disorders. Development of such a protocol has involved active interaction,
discussion and debate with patient support groups, the professional clinical care-providing
fraternity, and the international RDD research community.
The Colorectal Cancer Research Consortium (under the directorship of RR) had previously
described a substantial burden of disease along the west coast of South Africa, due to a
founder mutation. Interesting ‘anthropogenetic’ work revealed that the mutation arose some
18 generations ago. Further work in this homogeneous cohort showed that a range of
additional genetic changes (modifiers) influenced the age of onset and progression of
disease. A comprehensive clinical gastroenterological screening programme in the Northern
Cape has been developed on the back of the genetics research and service.
Individuals shown to harbour disease-predisposing mutations are very important in testing a
range of variables. The project is in the final stages of implementing the South African arm of
the international CAPP (Concerted Action Polyp Prevention) study on diet-based
modification of cancer risk. This work (which is not directly linked to any pharmaceutical
company, but the British MRC) was attracted to South Africa because of the international
publicity around the large cohort of South African families with familial colorectal cancer, in
whom the predisposing genetic defect has been identified within the Division. This project is
evolving naturally into other familial cancers; this niche area is going to be extremely
valuable for South Africa. All of the above research has been supported by the MRC, Retina
South Africa, Cansa and THRIP.
The successes within the Foetal Alcohol-Related Research programme, established within
the high-risk communities in the Western Cape have matured to the point of applying a
substantial R01 grant through the National Institutes of Health (specifically the NIAAA).
Divisional Statistics

UCT Staff

Administrative and Secretarial Staff (1Xp/T)

GSH Personnel


GSH/UCT Personnel

NHLS Personnel


Honorary Staff


Students

PhD 5
Masters 5
Honours 3
Total 20

Research Fields and Staff

Professor Raj S. Ramesar
Head of Division and personal chair, Director: MRC Human Genetics Research Unit;
Director: Cansa’s Colorectal Cancer Research Consortium; genetics of psychiatric disorders;
genetics of retinal degenerative disorders, molecular genetics of complex disorders
Dr Rik de Decker
Senior Specialist: 22q Deletion syndrome; fetal alcohol syndrome; SANTA study (Aetiology
of Tetralogy of Fallot), breast cancer, HIV and amniocentesis
Associate Professor Jacquie Greenberg
Head of Molecular Genetic Services: retinal degenerative disorders; genetics of neurological
disorders; neuromuscular disorders
Associate Professor Bongani Mayosi
molecular genetics of inherited cardiomyopathies
Dr S. Bardien
Postdoctoral fellow; genetics of hypertension and complex disorders
Emeritus Professor Peter Beighton
genetics of connective tissue disorders
Dr R. Goliath
Specialist scientist; genetics of neuromuscular diseases
Dr L. Matolweni
Postdoctoral fellow; genetics of cardiovascular diseases
Dr Ekow Oppon
Postdoctoral fellow; development of bioinformatic tools for the identification of disease
susceptibility genes

Dr George Rebello
Scientific Officer: bioinformatics; retinal degenerative disorders (RP17); development of a
genomics biotechnological platform
Ronald Smart
Head of Cytogenetic Service; chromosomal rearrangements and the 22q deletion syndrome
Professor Denis Viljoen (Honorary Research Associate)
Fetal Alcohol Syndrome

Divisional Contact Details

Postal Address: Division of Human Genetics, Department of Clinical Laboratory Sciences,
Faculty of Health Sciences, University of Cape Town, 7925, Observatory
Telephone: SA (21) 406 6297
Fax: SA (21) 447 7703
E-mail: [email protected]
CLINICAL LABORATORY SCIENCES
DIVISION OF IMMUNOLOGY
(Including the MRC/UCT Immunology of Infectious Diseases Unit)

Head of Division (Acting): Professor Lafras Steyn

Divisional Profile
2004 has seen our MRC Unit on “Immunology of Infectious Disease characterized by high
productivity with 18 international peer reviewed publications. We have maintained and
extended our excellent international and local reputation in infectious diseases as well as
gene targeting technology. Some of our mouse models are unique world wide, which
explains a high proportion of international funding due to much foreign collaboration. The
director is currently involved in seventy plus international collaborations, most of them highly
productive and successful.
Excitingly an MRC evaluation of “excellent” resulted in a renewal of our unit status for
another five year cycle. As a member of UCT’s new Institute for Infectious Immunology and
Molecular Medicine (IIDMM), we recently moved to new laboratories with expanded space
and a vibrant scientific environment.
Major breakthrough and success resulted from the first inducible and cell type-specific gene
deficient mouse models ’Made in South Africa’ published in the high impact journal -
Immunity (Herbert et al, 2004) and the – Journal of Immunology (Seki et al, 2004).
We succeeded with a new technological approach to autovaccinate, to protect against
autoimmune and infectious diseases. We were able to cure experimental autoimmune
encephalitis (Uyttenhove, et al, 2004), a model for multiple sclerosis and protected against
cutaneous leishmaniasis (Arendse et al, 2005).

In tuberculosis we demonstrated that adaptive immunity to mycobacterial infection is not
necessarily dependent on innate immunity, induced by Toll like receptor, published in the
high impact – Journal of Clinical Investigation (Fremond et al, 2004).
Impact on research output
These unique mouse models are applicable in many biological fields with our focus on
experimental models for human diseases.
Funding/Grants: As a consequence, we were again able to attract substantial financial
support from the USA (NIH/SCOR), Belgium (Flemish/NRF co-operation, Germany
(DFG/BMZ/NRF co-operation, France (PICS, UK (The Wellcome Trust), NRF (South Africa)
in addition to significant (10% of total income) MRC support.
Capacity development / strengthened research/ Collaboration
Having established a “world class” Division and MRC unit with a productive and vibrant “mix”
of International and national researchers has been satisfying. Highly reputed and well-known
scientists from across the globe are visiting us and broaden and challenge our minds with
their high caliber ideas. PAUSET guidelines were followed. An exciting achievement is the
recently awarded Bilateral Program grant from the Royal Society, UK and NRF, SA on the
“Immunology in parasitic diseases”, with strong capacity development and exchange
program for previously disadvantaged students.
Population group and gender composition:
More than 50% of our team members are from previously disadvantaged backgrounds and
50% to 60% are female.
Divisional Statistics
Permanent and Long-term Contract Staff


Students

Postdoctoral 5
Doctoral 12
Masters 4
Other 4
Total 25

Research Fields and Staff

F. Brombacher
the role of cytokines in human infectious diseases; vaccine and drug development in African
Trypanosomiasis, tuberculosis, Leishmaniasis, Bilharzia and other Helminth infections; the
macrophage effector killing genes in Listeriosis and Tuberculosis
B. Ryffel
TB; HIV; vaccine
P. Potter
allergic responses
A. Lopata
identification specific allergens; Anasikis pegreffi
P. Burger
fibroblast growth factor; prostatic stem cells ID
E.L. Wilson
balance VEGF/angiopoietin expression
E. du Toit
transplantation, population genetics; HLA
B. Nurse
cytokine profiles D. Herbert analysis of cell-specific IL-4 receptor alpha knock out mice in immunity against Schistosoma mansoni S. Magez Trypanosomiasis (human sleeping sickness) M. Radwanska Trypanosomiasis (human sleeping sickness) A. Marion cytokines and stress proteins involved in TH1 versus TH1 responses in atopy N. Nieuwenhuizen host protective Immunity to the food born parasite ANISAKIS Pegreffi with focus on the immunoregulatory role of IL-4 and IL-13 using IL-4α receptor deficient mice D. Georg detection of aerosolized seafood allergens in environmental samples from a seafood processing factory R. Baartjies occupational risk factors and interventions for reducing the incidence of allergy and asthma among bakery workers M. Drennan TB/Trypanosomiasis W. Horsnell Nippostrongylus brasiliensis infection R. Guler investigate using 4 different pathogens, i.e. Listeria monocytogenes, mycobacteria tuberculosis, mycobacteria bovis BCG, and Leishmania major S Joseph Schistosoma Mansoni; identification of genes involved in type2 granuloma formation A. Cutler role of IL-4 /13 in Leishmania Associate Professor Gordon Brown innate immunology Janet Willment Postdoctoral fellow; characterization of the Human β-Glucan Receptor, Dectin-1 Kevin Dennehy Postdoctoral fellow; Dectin-1 signal transduction pathways Elwira Pyz Postdoctoral fellow characterization of the Myeloid Inhibitory C-Type Lectin Receptor, MICL
Vicky Tsoni
Postdoctoral fellow; role of Dectin –1 in fungal infection
Cristal Huysamen
characterization of novel C-type lectin receptors
Research Associates
Dr Professor Bernhard Ryffel
TB; HIV; vaccine
Distinguished Visitors

Mary Phillips
Wel come Trust
Hans Haagen
Welcome Trust
Howard Engers
AHRI, Addis Ababa, Ethiopia Gerhard Walzel University of Stellenbosch Kim Taversham University of the Witwatersrand Stefan Kaufmann London School, UK HIV/TB Workshop 1 – 7 December 2004 Martin Bachman Cytos Biotech, Zurich, Switzerland Louis Montaner NIV SA

Divisional Contact Details

Postal Address: Division of Immunology, IIDMM, Level One, Room S 1.27, Wernher Beit South, Medical School, Observatory, 7925 Telephone: SA (21) 406 6616/6147 Fax: SA (21) 406 6029 E-mail: [email protected]






CLINICAL LABORATORY SCIENCES
DIVISION OF MEDICAL BIOCHEMISTRY

(Including the MRC/UCT Oesophageal Cancer Research Group and the MRC/UCT
Research Group for Receptor Biology)

Head of Division: Professor M. Iqbal Parker
Divisional Profile

The Division of Medical Biochemistry is a preclinical, basic science division in the UCT
Faculty of Health Sciences. Our principal functions are teaching and research.
Undergraduate teaching is in medical biochemistry to MBChB students. Postgraduate
teaching includes a vigorous Honours course (within the School of Biomedical Sciences) and
MSc and PhD programmes. Our research activities fall into 5 major areas: (1) Regulation of
collagen gene expression; (2) proteolytic processing of membrane proteins; (3) cellular and
molecular biology of breast and oesophageal cancer; (4) endocytic processing with particular
emphasis on mechanisms for intracellular survival of mycobacteria; (5) molecular
reproductive endocrinology. The Division also houses the Oesophageal Cancer Research
Group funded by the MRC. The Group includes researchers from UCT, UNITRA,
Stellenbosch and the MRC with Professor Parker as the Director. Professor Parker
continues to run the U.N.E.S.C.O. Molecular and Cell Biology Network laboratory, which
allows access of UNESCO funds for training of scientists from developing countries.
Professor Thilo has ongoing collaborations on mycobacterial research with groups in France
and Germany. A/Professor Arieh Katz and Dr Colleen Flanagan are co-directors of the
MRC/UCT Research Group for Receptor Biology and still has a very active collaboration with
Professor Millar who is currently Director of the MRC Reproductive Biology Unit in the United
Kingdom. The Division also regularly hosts researchers from other developing countries for
training in specialised techniques.
Divisional Statistics
Permanent and Long-term Contract Staff

Professors
Total 20

Honorary
Students

Doctoral
Undergraduate 374
Total 398

Research Fields and Staff
Permanent Staff

Professor M. Iqbal Parker
Head of Division and Director Research Operations; cel ular and molecular biology breast
and oesophageal cancer; collagen gene regulation
A/Professor Lutz Thilo
endocytic and membrane trafficking; mycobacterial phagosomes
A/Professor Arieh Katz
molecular reproductive endocrinology
Dr Cynthia Sikakana
Senior Lecturer; drug and xenobiotic metabolism
Dr Denver Hendricks
Senior Lecturer; oesophageal and cervical cancer
Dr Edward Sturrock
Wel come Trust Senior Fellow; structure/function studies on angiotensin-converting enzymes
Honorary Staff

Professor R.P. Millar
Honorary Professor – Director of MRC Reproductive Biology Unit, Edinburgh
Dr Edward Sturrock
Honorary Senior Lecturer; structure/function studies on angiotensin-converting enzymes
Dr Colleen Flanagan
Honorary Senior Lecturer; G protein – coupled receptors
Dr Henry Jabbour
Honorary Senior Lecturer; Cyclooxygenases in cancer
Scientific Officers

Dr Tommy Haylett
Research Officer; endocytic processing
Sylva Schwager
Senior Scientific Officer; proteolytic processing of membrane proteins

Postdoctoral Fellows

Dr Bo Wang
Identification of genes involved in susceptibility to oesophageal cancer
Dr Bernhard J. Fromme
Interaction of HIV-1 subtype C gp120 with LCRS chemokine receptor
Dr Sharon Prince
The role of TBX2 in the cell cycle and cancer
Dr Collet Dandara
Genetic polymorphisms in oesophageal cancer
Dr Virna Leaner
Regulation of collagen gene expression
Dr Trudie van Rensburg
Expression of hypoglycosylated angiotensin converting enzyme
Dr Anne Smith
Synthesis of 3-prolyl substituted lisinopryl inhibitors
Dr Aloysius Nchinda
The computer modelling and synthesis of C-domain selective ACE inhibitors
Dr Shaun Cochrane
Internalisation of the type 2 GNRH Receptor

Divisional Contact Details
Postal Address: Division of Medical Biochemistry, University of Cape Town, Faculty of Health Sciences, Anzio Road, Observatory, 7925 CLINICAL LABORATORY SCIENCES
DIVISION OF MEDICAL MICROBIOLOGY

Head of Division: Professor Lafras M. Steyn
Divisional Profile
Members of the division of Medical Microbiology continue to provide diagnostic services,
undergraduate and postgraduate medical student training, conduct research and provide
training for science students interested in a research career.
The composition of the Division is complex with staff drawn from the University of Cape
Town, Groote Schuur Hospital and Red Cross Children’s Hospital and National Health
Laboratory Service (NHLS). The microbiology diagnostic laboratories are located at the two
hospitals, while the molecular research laboratories are located in the Institute of Infectious
Diseases Building, Wernher Beit South, Health Sciences Campus.
Aside from providing a full range of diagnostic services, the division is involved in clinical and
diagnostic trials, investigating outbreaks of infection, and in defining the epidemiology of
various infectious agents. Important areas of research focus on tuberculosis, bacterial
antibiotic resistance, campylobacter infections and nosocomial pathogenes. A major effort is
directed into developing novel vaccines and vaccine vectors. The division also has a highly
cited site on the World Wide Web.
Divisional Statistics

Permanent and Long-term Contract Staff

Professors 1
Associate Professors

Honorary Staff

Honorary Lecturers


Students
Postdoctoral 4
Registrars 2
Doctoral 4
Masters 3
Honours 10
Undergraduate 180
Total 203


Research Fields and Staff

Permanent Staff
Professor Lafras M. Steyn
molecular biology of mycobacterium tuberculosis
Dr Gay Elisha
antibiotic resistance mechanisms and molecular epidemiology
Dr Steve Oliver
infection control policies and antibiotic usage
Dr Andrew Whitelaw
development of new diagnostic assays for tuberculosis
Registrars, Joint Appointments

Dr Sumaya Haffejee
Dr Colleen Bamford
Honorary Staff
Dr H. Hoppe
Protozoan parasites
Associate Professor Albert Lastovica
Campylobacter and Helicobacter
Dr Linda Gail Bekker Immunopathogenesis of tuberculosis Dr Marc Mendelson
Innate immune response to mycobacterium tuberculosis
Contract Research Staff

Dr Jenny Ramesar
Human papillomavirus typing
Dr Mark Nicol
Phenotypic and genotypic characterisation of clinical strains in Mycobacterium tuberculosis
causing pulmonary and extra-pulmonary disease in children
Heidi Segal
Carbapenen resistance in Pseudomonas aeruginosa

Postdoctoral Fellows
Dr Ros Chapman
mycobacterial stress responses
Dr Karen Shires
coldshock in mycobacteria
Dr Gavin Rush
attenuation of gene expression in mycobacteria
Dr Lemese Ah Tow
development of novel diagnostic methods for tuberculosis
Divisional Contact Details
Postal Address: Division of Medical Microbiology, University of Cape Town, Medical School,
Anzio Road, Observatory, 7925
Telephone: SA (21) 406 6363
Fax: SA (21) 448 8153
E-mail: [email protected]
Webpage: http://www.uct.ac.za/depts/mmi/index.html CLINICAL LABORATORY SCIENCES
DIVISION OF MEDICAL VIROLOGY
Head of Division: Professor Girish J. Kotwal
Divisional Profile

The Division of Medical Virology consists of three major research groups, namely, Viral
vaccines group, HIV diversity and pathogenesis group and the viral biotechnology group.
In mid-2004, the research groups after several decades in the basement and in the Wernher
and Beit North have moved to the newly renovated Wernher and Beit South top floor. The
clinical virology which was located in Wernher and Beit North for decades moved to the
Groote Schuur Hospital under the NHLS umbrella.
During 2004, Associate Professor Annalise Williamson was promoted to Professor. All the
three research groups continue to receive funding from several different sources including
SAAVI, DST Innovation Fund, CIPRA, Wellcome Trust, Doris Duke etc. In addition, the
Division received funding for the purchase of a major piece of equipment to perform protein
interaction studies. Members of the Division have been very active in the IIDMM
administration, serving on the membership committee, the research committee, the finance
committee and the DMAG.
Members of the division were also active in organising conferences at the Wolfson Pavilion,
namely “Introduction of new vaccines into the Expanded program on Immunization (EPI) in
Southern Africa: public health, educational, and economical aspects”, the first international
conference on natural products and molecular therapy.
Divisional Statistics

Permanent and Long-term Contract Staff
Professors 2
Associate Professors
Lecturers 1 Research and Technical Support Staff Total 52
Honorary Staff

Honorary Lecturers

Students

Postdoctoral 9
Masters 7
Doctoral 13
Total 29


Research Fields and Staff
Permanent Staff
Professor Girish J. Kotwal
viral evasion of host defense; drug discovery
Professor Anna-Lise Williamson
Human Papillomaviruses; HIV-1 vaccine development
Associate Professor Carolyn Williamson
HIV molecular biology; HIV-1 vaccine development
Dr Diana Hardie
molecular biology of Hepatitis B Virus - management of diagnostics viral lab
Dr Jane Yates
management of diagnostic virology laboratories; Parvovirus B19, viral infections in the
immunocompromised
Dr Diane Marais
Human Papillomavirus Immunology
Dr Heidi Smuts
Hepatitis C; Novel viruses (TT virus, human metapneumovirus); infectious agents in
oesophageal cancer
Lecturers
Dr Jo-Ann Passmore
Registrars, Joint Appointments

Dr Marvin Hsiao
molecular diagnostics
Dr Craig Corcoran
molecular diagnostics

Honorary Staff

Dr Tim Tucker
Senior Lecturer; HIV vaccine development
Dr Wolfe Katz
vaccine development

Contract Research Staff

Mr. Bruce Allan
diagnosis of human papillomavirus
Dr William Bourn
HIV Vaccines
Mrs. Helba Bredell
HIV
Mrs. Debbie de Villiers
Human Papillomavirus
Mr. Jandre Grobler
HIV
Mr. Rodney Lucas
HPV Vaccines
Mr. Baraka (Lucky) Malaza
HIV
Ms Marleze Rheeder
HIV Vaccines
Ms Yolanda Truter
HIV Vaccines
Mrs. Tracey Mul er
HIV
Mr. Craig Adams
HIV Vaccines; HIV Diversity
Ms Florette Treunicht
Acute infection with HIV-1 subtype C study (CAPRISA)
Dr Joanne van Harmelen
HIV vaccine development (SAAVI); HIV diversity (CAPRISA)
Distinguished Visitors

Dr Mary Phillips (Wellcome Trust - UK)
Dr Wolfgang Preiser (University of Frankfurt)
Dr Barry Rouse (University of Tennessee)
Dr Gary Cohen (University of Pennsylvania)
Postdoctoral Fellows
Dr Wendy Burgers
HIV Vaccines

Dr Vandana Govan
HPV
Dr Darren Marten
HIV Recombination
Dr Helen Stutz
HIV BCG Vaccines (SAAVI)
Dr Mbulelo Maneli
HIV Diversity
Dr Makobetsa Khati
Aptamers

Divisional Contact Details
Postal Address: Division of Medical Virology, Wernher and Beit Building South Wing,
University of Cape Town, Medical School, Anzio Road, 7925
Telephone: SA (21) 406 6126
Fax: SA (21) 406 6018
Webpage: http://www.uct.ac.za/depts/mmi/index.html
CLINICAL LABORATORY SCIENCES

Research Output

(Including the following Divisions/Institutes: Chemical Pathology (including the Chemical Pathology
sections of Groote Schuur Hospital and Red Cross War Memorial Children’s Hospital), Forensic
Medicine and Toxicology (including the Gender, Health and Justice Research Unit), Haematology,
Human Genetics (including the MRC/UCT Human Genetics Research Unit and the CANSA Colorectal
Cancer Research Consortium), Immunology (including the MRC/UCT Immunology of Infectious
Diseases Unit), Medical Biochemistry (including the MRC/UCT Oesophageal Cancer Research Group
and the MRC/UCT Research Group for Receptor Biology) Medical Microbiology and Medical Virology)
ARTICLES IN PEER-REVIEWED JOURNALS

Artz, L. 2004. Better safe than sorry: Magistrates’ views on the Domestic Violence Act. South
African Crime Quarterly, 7: 1-8.
Artz, L. 2004. Tough choices: Difficulties facing magistrates in applying protection orders.
South African Crime Quarterly, 8: 25-30.
Berman, M.C. and Karlish, S.J. 2003. Interaction of an aromatic dibromoisothiouronium
derivative with the Ca2+- ATPase of skeletal muscle sarcoplasmic reticulum. Biochemistry,
42: 3556-3566.
Billings, B., Smith, S.A., Zhang, Z., Lahiri, D.K. and Kotwal, G.J. 2004. Lack of N1L gene
expression results in a significant decrease of vaccinia virus replication in mouse brain.
Annals of New York Academy of Science, 1030: 297-302.
Brombacher, F. 2004. Anti IL - 12 auto - vaccination: A treatment for experimental
autoimmune encephalomyelitis at the expense of reduced cell - mediated immunity. Journal
of Immunology, 34: 3572-3581.
Brombacher, F. 2004. IL - 4 confers protection against the early acute phase of
Cryptosporidium parvum infection by promoting Th 1 cytokine expression. Journal of
Infectious Diseases, 190: 1019-1025.
Brombacher, F. 2004. IL-4/IL-13-activated alternative macrophages are essential to survive
schistosomiasis and downmodulate Type 1 responses and immunopathology. Immunity, 20:
623-635.
Brombacher, F. 2004. Intestinal pathology during acute toxoplasmosis is IL - 4 dependent
and unrelated to parasite burden. Parasite Immunology, 26: 75-82.
Brombacher, F. 2004. Mice deficient in interleukin-4 (IL-4) or IL-4 receptor alpha have higher
resistance to sporozoite infection with plasmodium berghei (ANKA) than do naive wild-type
mice. Infection and Immunity, 72(1): 322-331.
Brombacher, F. 2004. P75 Tumor necrosis factor-receptor shedding occurs as a protective
host response during African Trypanosomiasis. Journal of Infectious Diseases, 189: 527-
539.
Brombacher, F. 2004. Pinpointing IL-4-independent and IL-4-influenced acquisition and
maintenance if Th2 activity by CD4 T cells. European Journal of Immunology, 34: 686-694.
Brombacher, F. 2004. Role of IL-4-induced GATA-3 expression as a time-dependent instruction switch on cytokine expression in helper T cell differentiation. Journal of Immunology, 174: 6158-6166. Chubb, A., Schwager, S.L.U., Van der Merwe, E.L., Ehlers, M.R.W. and Sturrock, E.D. 2004. Deletion of the cytoplasmic domain increases basal shedding of angiotensin-converting enzyme. Biochemical and Biophysical Research Communications, 314: 971-975. Davies, J.Q., Hall, P., Kaschula, R.O.C., Sinclair-Smith, C.C., Hartley, P.S., Rode, H. and Millar, A.J.W. 2004. Hepatoblastoma - Evolution of management and outcome and significance of histology of the resected tumor. A 31-year experience with 40 cases. Journal of Pediatric Surgery, 39(9): 1321-1327. DiPerna, G., Stack, J., Bowie, A.G., Boyd, A., Kotwal, G.J., Zhang, Z., Arvikar, N.K., Latz, E., Fitzgerald, K.A. and Marshall, W.L. 2004. Poxvirus protein N1L targets the I-kappaB kinase complex, inhibits signaling to NF-kappaB by the tumor necrosis factor superfamily of receptors, and inhibits NF-kappaB and IRF3 signalling by toll-like receptors. Journal of Biological Chemistry, 279(35): 36570-36578. Ganesh, V.K., Smith, S.A., Kotwal, G.J. and Murthy, K.H. 2004. Structure of vaccinia complement protein in complex with heparin and potential implications for complement regulation. Proceedings of the National Academy of Sciences of the United States of America, 101(24): 8924-8929. Gordon, K., Redelinghuys, P., Schwager, S.L.U., Ehlers, M.R.W., Papageorgio, A.C., Natesh, R., Acharya, K.R. and Sturrock, E.D. 2003. Deglycosylation, processing and crystallization of human testis angiotensin converting enzyme. Biochemical Journal, 371: 437-442. Govender, D., Ramdial, P.K., Clark, B. and Chetty, R. 2004. Clear cell (glycogen-rich) gastric adenocarcinoma. Annals of Diagnostic Pathology, 8(2): 69-73. Govender, D., Sing, Y. and Chetty, R. 2004. Sertoli cel s nodules in the undescended testis: A histochemical, immunochistochemical, and ultrastructural study of hyaline deposits. Journal of Clinical Pathology, 57(2): 802-806. Grobler, J., Gray, C.M., Rademeyer, C., Seoighe, C., Ramjee, G., Karim, S.A., Morris, L. and Williamson, C. 2004. Incidence of HIV-1 dual infection and its association with increased viral load set point in a cohort of HIV-1 subtype C-infected female sex workers. Journal of Infectious Diseases, 190(7): 1355-1359. Hadley, G.P., Govender, D. and Landers, G. 2004. Primary tumours of the liver in children: An African perspective. Pediatric Surgery International, 20: 314-318. Hammersma, H., Gardner, J. and Beighton, P. 2003. The natural history of sclerosteosis. Clinical Genetics, 63: 191-196. Hassan, H., Toerien, A. and Potter, P.C. 2003. Evaluation of immunotherapy for seasonal and perennial allergic rhinitis. Current Allergy and Clinical Immunology, 16(11): 22-23. Heath, L.E., Martin, D., Warburton, L., Perrin, M., Horsfield, W., Kingsley, C., Rybicki, E.P. and Williamson, A.-L. 2004. Evidence of unique genotypes of Beak and Feather Disease Virus in southern Africa. Journal of Virology, 78(17): 9277-9284. Hesseling, P.B., Harley, E.H., Zietsman, L., Van Lill, S., Preston-Martin, S. and Wessels, G. 2004. Incidence of acute lymphoblastic leukaemia in white and coloured children in the Western Cape. South African Medical Journal, 94(7): 533-536. Ho, V.C., Gupta, A., Kaufmann, R. Todd, G., Vanaclocha, F., Takaoka, R., Fölster-Holst, R., Potter, P., Marshall, K., Thurston, M., Bush, C. and Cherill, R. 2003. Safety and efficacy of nonsteroid pimecrolimus cream 1% in the treatment of atopic dermatitis in infants. Journal of Pediatrics, 142: 155-162. Jaffray, A., Shephard, E.G., Van Harmelen, J., Williamson, C., Williamson, A.-L. and Rybicki, E.P. 2004. Human immunodeficiency virus type 1 subtype C Gag virus-like particle boost substantially improves the immune response to a subtype C gag DNA vaccine in mice. Journal of General Virology, 85(2): 409-413. Jeebhay, M., Robin, T.G. and Lopata, A.L. 2004. World at work: Fish processing workers. Occupational and Environmental Medicine, 61(5): 471-474. Lastovica, A.J. 2003. Molecular typing of Campylobacter jejuni and Campylobacter coli. Journal of Clinical Microbiology, 41(3): 1349-1350. Lastovica, A.J. 2003. Optimal detection of Campylobacter spp in stools. American Journal of Clinical Pathology, 56: 480. Lastovica, A.J. 2003. Prevalence and optimal detection of C.upsaliensis in stool specimens. Clinical Infectious Diseases, 36: 1624-1625. Lastovica, A.J. and Le Roux, I.M. 2003. Serious Thrombocytopenia due to Dengue Hemorrhagic fever treated with high dosages of immunoglobulin. Clinical Infectious Diseases, 36(June): 1623. Lemmer, E.R., Vessey, D., Gelderblom, W.C.A., Shephard, E.G., Van Schalkwyk, D.J., Van Wijk, R.A., Marasas, W.F.O., Kirsch, R.E. and Hall, P. 2004. Fumonisin B1-induced hepatocellular and cholangiocellular tumors in male Fischer 344 rats: Potentiating effects of 2-acetylaminofluorene on oval cell proliferation and neoplastic development in a discontinued feeding study. Carcinogenesis, 25(7): 1257-1264. Lopata, A.L. 2004. Adverse reactions to fish. Current Allergy and Clinical Immunology, 17: 4-8. Lopata, A.L. 2004. Evaluating allergic responses to bird allergens. Current Allergy and Clinical Immunology, 17(2): 87-90. Lopata, A.L. 2004. Qualitative and quantitative evaluation of bird specific IgG antibodies. International Archives of Allergy and Immunology, 134(2): 173-178. Lopata, A.L., Jeebhay, M., Baatjies, R. and Thrower, S.J. 2004. Occupational allergies in the seafood industry - a comparative study of Australian and South African workplaces. International Maritime Health, 55(4): 61-72. Lopata, A.L., Schinkel, M., Potter, P.C., Jeebhay, M., Hashemi, C., Johansson, S.G.O. and Van Hage-Hamsten, M. 2004. Qualitative and quantitative evaluation of bird specific IgG antibodies. International Archives of Allergy and Applied Immunology, 134: 173-178. Loveland, B.E., Williams, L., Baker, L., Brandon, M.R., Xing, P.X., Kahn, D., McKenzie, I.F.C., Milland, J., Kyriakou, P., Thorley, B.R., Christiansen, D., Lanteri, M.B., Van Regensburg, M., Duffield, M. and French, A.J. 2004. Characterization of a CD46 transgenic pig and protection of transgenic kidneys against hyperacute rejection in non-immunosuppressed baboons. Xenotransplantation, 11: 171-183. Mall, A.S. and Taylor, K.L. 2004. The relationship of a Helicobacter Heilmanii infection to the mucosal changes in abattoir and laboratory pig stomach. Surgery Today, 34: 943-949. Mangisa, N.P., Kew, M.C., Smuts, H., Kramvis, A., Linley, W., Skelton, M., Tucker, T., Hall, P., Kahn, D. and Jilbert, A.R. 2004. Molecular characterisation of duck hepatitis B virus isolates from South African ducks. Virus Genes, 28(2): 179-186. McIntosh, D.B., Clausen, J.D., Vilsen, B., Einholm, P. and Andersen, J.P. 2004. Glutamate-183 in the conserved TGES motif of domain A of sarcoplasmic reticulum Ca2+-ATPase assists in catalysis of E2/E2P partial reactions. Biochemistry, 101(9): 2776-2781. Morgan, K., Conklin, D., Pawson, A.J., Sellar, R., Ott, T.R. and Millar, R.P. 2003. A transcriptionally active human typeII Gonadotropin-releasing hormone receptor gene homolog overlaps two genes in the antisense orientation on chromosome 1q.12. Endocrinology, 144(2): 423-436. Morganroth, J., Potter, P.C. and Taglietela, M. 2003. Consensus group on new generation antihistamines (CONGA). S.T. Holgate: Panel of cardiotoxity. Clinical Experimental Allergy, 33: 1305-1324. Mossmann, H., Claussen, B., Fvrster, I., Brombacher, F., Holscher, C., Mohrs, M., Arendse, B., Schwegmann, A.R., Radvanska, M.B., Kirsch, R. and Hall, P. 2004. Alternative macrophage activation is essential for survival during Schistosomiasis and downmodulates T Helper 1 responses and immunopathology. Immunity, 20: 623-635. Novitzky, N., Thomas, V., Abrahams, L., Du Toit, C.E. and McDonald, A.B. 2004. Increasing dose intensity of Anthracycline antibiotics improves outcome in patients with acute Myelogenous Leukemia. American Journal of Hematology, 76(4): 319-329. Novitzky, N., Thomas, V., Hale, G. and Waldmann, H. 2004. CAMPATH-1 Antibodies "In the Bag" for haematological malignancies: The Cape Town experience. Cytotherapy, 6(2): 172-181. Novitzky, N., Thomas, V., Stubbings, H., Hale, G. and Waldmann, H. 2004. Radiotherapy-based conditioning is effective immunosuppression for patients undergoing transplantation with T-cell depleted stem cell grafts for severe aplasia. Cytotherapy, 6(5): 450-456. Nuttall, J., Wilmshurst, J., Ndondo, A., Yeats, J., Corcoran, C., Hussey, G.D. and Eley, B.S. 2004. Progressive multifocal leukoencephalopathy after initiation of highly active antiretroviral therapy in a child with advanced human immunodeficiency virus infection: A case of immune reconstitution inflammatory syndrome. The Pediatric Infectious Disease Journal, 23(7): 683-685. Orren, A. and Potter, P.C. 2004. Complement component C6 deficiency and susceptibility to Neisseria Meningitidis infection. South African Medical Journal, 95(5): 345-346. Papp, K., Staub, D., Harper, J., Potter, P.C., Puig, L., Ortonne, J.P., Molloy, S., Barbier, N. and Paul, C. 2004. Effect of Pimecrolimus Cream 1% on the long-term course of paediatric atopic dermatitis. International Journal of Dermatology, 43(12): 978-983. Patel, M., Andronikou, S., Solomons, R., Sinclair, P. and McCulloch, M. 2004. Idiopathic arterial calcification in childhood. Pediatric Radiology, 34: 652-655. Pawson, A.J., Maudsley, S.R., Lopes, J., Katz, A.A., Sun, Y.M., Davidson, J.S. and Millar, R.P. 2003. Multiple determinants for rapid agonist-induced internalization of a nonmammalian gonadotropin-releasing hormone receptor: A putative palmitoylation site and threonine doublet within the carboxyl-terminal tail are critical. Endocrinology, 144(9): 3860-3871. Pillay, K., Solomons, R., Daubenton, J.D. and Sinclair-Smith, C.C. 2004. Interdigitating dendritic cell sarcoma: A report of four paediatric cases and review of the literature. Histopathology, 44(3): 283-291. Pillay, N.S., Kellaway, L.A. and Kotwal, G.J. 2004. Molecular mechanisms, emerging etiological insights and models to test potential therapeutic interventions in Alzheimer's disease. Current Alzheimer Research, 1: 295-306. Post, F.A. and Steyn, L.M. 2004. Genetic polymorphism in Mycobacterium tuberculosis isolates from patients with chronic multidrug-resistant tuberculosis. Journal of Infectious Diseases, 190: 99-106. Potter, P.C. 2003. Levocetirizine is effective for symptom relief including nasal congestion in adolescent and adult (PAR) sensitized to house dust mites. Allergy, 58: 893-899. Potter, P.C. 2003. The increase in prevalence of asthma and atopy in Africans. Asthma Acta, 4: 57-58. Potter, P.C., Van Niekerk, C.H. and Scheepers, J.M. 2003. Fexofenadine does not impair reaction time, decision making and driver behaviour. Annals of Allergy, 91: 177-181. Potter, P.C., Van Niekerk, C. and Schoeman, H.S. 2003. Triamcinolone Acetonide (Nasacor AQ) improves quality of life in persistent allergic rhinitis. Annals of Allergy, 91(4): 368-374. Rademeyer, C., Van Harmelen, J.H., Ramjee, G., Karim, S.S. and Williamson, C. 2004. Heterosexual transmission of multiple highly conserved viral variants in HIV-1 subtype C-infected seronegative women. AIDS, 18(15): 2096-2098. Rajput, J., Moss, J.R., Hutton, A.T., Hendricks, D.T., Arendse, C.E. and Imrie, C. 2004. Synthesis, characterization and cytotoxicity of some palladium(II), platinum(II), rhodium(I) and iridium(I) complexes of ferrocenylpyridine and related ligands. Crystal and molecular structure of trans-dichlorobis (3-ferrocenylpyridine) palladium(II). Journal of Organometallic Chemistry, 689: 1553-1568. Ramburan, R.M., Chetty, R., Hadley, G.P., Naidoo, R. and Govender, D. 2004. Microsatellite analysis of the DCC gene in nephroblastoma: Pathologic correlations and prognostic implications. Modern Pathology, 17: 89-95. Rebello, M.T., Bonapace, G., Waheed, A., Shah, G.N., Sly, S., Ramesar, R., Vorster, A.A., Roberts, L.J., Ehrenreich, L.S., Oppon, E., Gama, A.D., Bardien, S. and Greenberg, L.J.H.L. 2004. Apoptosis-inducing signal sequence mutation in carbonic anhydrase IV identified in patients with the RP17 form of retinitis pigmentosa. Proceedings of the National Academy of Sciences of the United States of America-Biological Sciences, 101: 6617-6622. Ronacher, K., Matsiliza, N., Nkwanyana, N., Pawson, A.J., Adam, T., Flanagan, C.A., Millar, R. and Katz, A.A. 2004. Serine residues 338 and 339 in the carboxyl-terminal tail of the type II gonadotropin-releasing hormone receptor are critical for ß-arrestin-independent internalization. Endocrinology, 145(10): 4480-4488. Rousseau, J., Pokorny, L., Glaser, J. and Creemers, P.C. 2003. Presisposing and protective HLA-DR and DQ al eles for rheumatoid arthritis in South African mixed-ancestry and Xhosa populations. South African Journal of Science, 99: 89-92. Rousseau, J., Williams, R.C. and Creemers, P.C. 2003. HLA DRB1, DQA1, DQB1 and DPB1 polymorphisms in Namibian Khoi and San and in the Xhosa and South African mixed-ancestry population. South African Journal of Science, 99: 81 - 89. Rush, G. and Steyn, L.M. 2004. Translation enhancement by optimized downstream box sequences in Escherichia coli and Mycobacterium smegmatis. Biotechnology Letters, 27(3): 173-179. Ryffel, B. 2004. Arthropod-derived histamine-binding protein prevents murine allergic asthma. Journal of Immunology, 173(5): 3281-3286. Ryffel, B. 2004. Fatal Mycobacterium tuberculosis infection despite adaptive immune response in the absence of MyD88. Journal of Clinical Investigation, 114(12): 1790-1799. Ryffel, B. 2004. Reduced local growth and spread but preserved pathogenicity of a{Delta}purC mycobacterium tuberculosis auxotrophic mutant in gamma interferon receptor-deficient mice after aerosol infection. Infection and Immunity, 73(1): 666-670. Ryffel, B. 2004. Toll-like receptor pathways in the immune responses to mycobacteria. Microbes and Infection, 6(10): 946-959. Ryffel, B. and Botha, T. 2003. Reactivation of latent tuberculosis infection in TNF-deficient mice. Journal of Immunology, 171(6): 3110-3118. Sabaratnam, R.M., Govender, D. and Reddi, A. 2004. Acinic cell carcinoma: An unusual cause of Bronchial obstruction in a child. Pediatric and Developmental Pathology, 7: 521-526. Segal, H., Elisha, B.G. and Nelson, E.C. 2004. Genetic environment and transcription of ampC in an Acinetobacter baumannii clinical isolate. Antimicrobial Agents and Chemotherapy, 48(2): 612-614. September, A., Vorster, A.A., Ramesar, R. and Greenberg, L.J.H.L. 2004. Mutation spectrum and founder chromosomes for the ABCA4 gene in South African Stargard Disease patients. Investigative Ophthalmology and Visual Science, 1(45): 1705-11. Smythe, D. 2004. Missed opportunities: Confiscation of weapons in domestic violence cases. South African Crime Quarterly, 10: 19-26. Snyman, J.R., Potter, P.C. and Levin, J. 2004. The effect of betamethazone and loratadine combination therapy on severe exacerbations of allergic rhinitis: A randomised controlled trial. Clinical Pharmacology. Clinical Drug Investigation, 24(5): 265-273. Steenkamp, D.J. and Vogt, R. 2004. Preparation and utilization of a reagent for the isolation and purification of low-molecular-mass thiols. Analytical Biochemistry, 325: 21-27. Steinman, H., Dawson, H., Kawalski, M., Toerien, M. and Potter, P.C. 2003. Bronchial
hyperresponsiveness and atopy in urban and rural South African children. Paediatric Allergy
and Clinical Immunology, 14: 383-393.
Van Zyl-Smit, R., Firth, J.C., Duffield, M. and Marais, A.D. 2004. Renal tubular toxicity of
HMG-CoA reductase inhibitors. Nephrology Dialysis Transplantation, 19: 3176-3179.
Wahn, U., Metlzer, E.O., Finn, A., Kowalski, M.L., De Costa, P., Hedlin, G., Bachert, C.,
Pinto, R., Bregna Cagnami, C., Potter, P.C., Simons, F.E.R. and Runtin, E. 2003.
Fexofenadine is efficacious and safe in children with seasonal allergic rhinitis. Journal of
Allergy and Clinical Immunology, 111: 763-769.
CHAPTERS IN BOOKS

Potter, P.C. 2003. Allergen immunotherapy. MIMS Disease Review: 1-5. Pinegowrie: MIMS.
Potter, P.C. 2003. An approach to rhinitis including allergic rhinitis. MIMS Disease Review:
27-35. Pinegowrie: MIMS.
Smythe, D. and Parenzee, P. 2004. Acting against domestic violence. In B. Dixon and E. van
der Spuy (Eds.), Justice gained? Crime and crime control in South Africa’s transition: 140-
162. Cape Town: University of Cape Town Press, Willan Publishers.
PEER-REVIEWED PUBLISHED CONFERENCE PROCEEDINGS
Masters, K. and Duffield, M. 2004. WebCT and Anatomical Pathology tutorials. Proceedings
of the 4th IEEE International Conference of Advanced Learning Technologies (ICALT2004),
Joensuu, Finland, 30 August - 1 September 2004, 1039-1043.
Rebello, M.T., Bonapace, G., Waheed, A., Shah, G.N., Sly, S., Ramesar, R., Vorster, A.A.,
Roberts, L.J., Ehrenreich, L.S., Oppon, E., Gama, A.D., Bardien, S. and Greenberg, L.J.H.L.
2004. An apoptosis-inducing signal sequence mutation suggests carbonic anhydrase IV
underlies the RP17 form of autosomal dominent retinitis pigmentosa. Proceedings of the XI
International Symposium on Retinal Degeneration, Australia, 6617-6622.
NON PEER-REVIEWED PUBLISHED CONFERENCE PROCEEDINGS
Mabizela, N., Rodgers, A., Sturrock, E.D. and Schwager, S.L. 2004. Analysis of urinary bikunin in calcium oxalate crystals from the black and white population groups in South Africa. Proceedings of 10th International Symposium on Urolithiasis. Kidney Stones: Inside and Out, Hong Kong, 85-86. Novitzky, N., Thomas, V., Stubbings, H., Hale, G. and Waldmann, H. 2004. Radiotherapy-based conditioning is effective immunosuppression for patients undergoing transplantation with T-cell depleted stem cell grafts for severe aplasia. Proceedings of 44th Annual Conference of the Federation of South African Societies of Pathology (FSASP), Stellenbosch University Campus, Cape Town, 92. PUBLISHED CONFERENCE ABSTRACTS

Clarkson, V., Hall, P., Shephard, E.G., Kirsch, R. and Marais, A.D. 2004. The ethanol
feeding increases CYP2E1 activity in the methionine-choline-deficient mouse model for
NASH. Liver International 2004 24: Supplement 4: 20.
Clarkson, V., Shephard, E.G., Kirsch, R., Marais, A.D. and Hall, P. 2004. Lipid peroxidation
is increased following endotoxin challenge in a rodent nutritional model of NASH. Liver
International 2004 24: Supplement 4: 20.
Craig, P. 2003. Training of parent support groups in basic counselling skills and genetic
knowledge. 10th Biennial Congress of the Southern African Society of Human Genetics: 33-
34.
Epstein, D., Locketz, M.L., Watermeyer, G. and Hall, P. 2004. The aetiology of oesophageal
ulceration in HIV infected patients in Cape Town, South Africa. South African Medical
Journal, 94(8): 686.
Fenemore, B., Lopata, A. and Potter, P.C. 2003. Evaluation of the Western Blot in allergy
testing. Current Allergy and Clinical Immunology, 16(3): 118.
Hall, P. 2004. A pilot study to evaluate the role of serum lipids and LDL particle size as in
disease severity in subjects with NAFALD. South African Medical Journal, 94(8): 682.
Hall, P. 2004. Correlation of body mass index (MBI) with the different types of non-alcoholic
fatty liver disease (NAFALD). South African Medical Journal, 94(8): 682.
Hall, P. 2004. Drug induced hepatitis (including herbs). Pathology International 2004
54(Suppl): 295-298.
Hall, P. 2004. Histological findings on liver biopsy in South African patients with non-
alcoholic fatty liver disease. South African Medical Journal, 94(8): 687.
Hall, P. 2004. The correlation of serum ALT level and AST/ALT ratio and the histological
types in patients with non-alcoholic fatty liver disease (NAFALD). South African Medical
Journal, 94(8): 676.
Hall, P., McLeod, H., Panieri, E., Elazzabi, T., Dent, D. and Mall, A.S. 2004. The expression
of mucin in gastric remnant carcinoma. A histological and immunohistochemical study. South
African Journal Surgery, 42:25.
Hawarden, D., Mileham, J. and Potter, P.C. 2003. Anaphylaxis: Rather a shock. Current
Allergy and Clinical Immunology, 16(3): 117.
Jeebhay, M., Baatjies, R., Lopata, A.L., Bateman, E.D. and Robin, T.G. 2004. Baker's
asthma in small bakeries of a supermarket chainstore in the Western Cape. SA Respiratory
Journal, 10(2): 71.
Jeebhay, M., Robins, T.G., Baatjies, R. and Lopata, A.L. 2004. Environmental exposure
characterization of fish processing workers in South Africa. X-2004 Conference on Exposure
Assessment in a changing environment. Tijdschrift voor toegepaste Arbowetenschap, S(02):
1.
Jeebhay, M., Robins, T.G., Baatjies, R. and Lopata, A.L. 2004. Relationship between in vivo and in vitro markers of IgE in relation to work-related allergic symptoms among seafood processing workers. Current Allergy and Clinical Immunology, 17(3): 138. Jeebhay, M., Robins, T.G., Baatjies, R. and Lopata, A.L. 2004. Relationship between in vivo and in vitro markers of IgE in relation to work-related allergic symptoms among seafood processing workers (abstract 1158). XXIII Congress of the European Academy of Allergology and Clinical Immunology, Amsterdam, June 2004 EAACI: Published on CD-Rom. Novitzky, N., Thomas, V., Davison, G.M., Thomson, J. and Abrahams, L. 2004. A different but cost-effective schedule for mobilization of CD34+ cells for transplantation. Bone Marrow Transplantation, 33(Suppl 1): S95. Novitzky, N., Thomas, V., Hale, G. and Waldmann, H. 2004. T-cell depletion of stem cell grafts with Campath-1H 'in the bag' followed by cyclosporin immunosuppression remains a cost effective strategy in transplantation. Bone Marrow Transplantation, 33(Suppl 1): S93. Nurse, B., Motala, C. and Potter, P.C. 2004. HDM-Stimulated IL13 Release in atopic asthmatic children with a high prevalence of Ascaris Infestation. Current Allergy and Clinical Immunology, 17(3): 138-139. Nurse, B., Motala, C. and Potter, P.C. 2003. Lymphocyte proliferation and IgE responses in atopic South African children with a high prevalence of Ascaris infestation. Current Allergy and Clinical Immunology, 16(3): 120. Obihara, C.C., Van Liu, S., Marais, B.J., Hesseling, A.C., Lawrence, K., Potter, P.C., Bateman, E., Gie, R., Kimpen, J. and Beyers, N. 2003. The association between atopy, asthma and tuberculin skin test in children living in an area with high prevalence of tuberculosis. Current Allergy and Clinical Immunology, 16(3): 134. Olarogun, J., Marais, D.J., Soule, S. and Van der Spuy, Z.M. 2004. Effects of weight loss on dyslipidaemia in polycystic ovary syndrome. 40th Congress of SEMDSA (Society for Endocrinology, Metabolism, and Diabetes of South Africa) in Durban: 29. Potter, P.C. 2003. The increase in the prevalence of asthma bronchial hyperreactivity and atopy in Africans. In R. Sepiashvili (Ed.), Asthma: from Genes to Clinical Management. St. Petersburg. International Proceedings and Diseases. Monduzzi 2003; 109-112. Potter, P.C., Lisanti, M., Groenewald, M., Portes, R. and Weinberg, E.G. 2004. Pollen aeroallergen sensitisation and efficacy of Fexofenadine in children from the Southern Hemisphere. Current Allergy and Clinical Immunology, 17(3): 139. Potter, C., Lisanti, M., Groenewald, M., Portes, R., Weinberg, E., Vucovich, P., Puterman, A., Baena-Cagnani, C., Hardy, P. and Ruuth, E. 2004. Pollen allergen sensitization and efficacy of Fexofenadine in children from the southern Hemisphere. Journal of Allergy and Clinical Immunology, 113(2): 5232. Prescott, R. and Potter, P.C. 2003. FACS Analysis: A potentially useful tool in Allergy diagnosis. Current Allergy and Clinical Immunology, 16(3): 118. Schinkel, M., Potter, P.C., Jeebhay, M., Hashemi, C., Johansson, S.G.O., Van Hage-Hamsten, M. and Lopata, A.L. 2004. A comparison of qualitative and quantitative evaluation of bird specific IgG anitibodies in exposed and non-exposed individuals (abstract 1193). XXIII Congress of the European Academy of Allergology and Clinical Immunology,
Amsterdam, June 2004 EAACI: Published on CD-Rom.
Steinman, H., Donson, H., Kawalski, M., Toerien, A. and Potter, P.C. 2003. Bronchial
hyperresponsiveness and atopy in urban, peri-urban and rural South African children.
Current Allergy and Clinical Immunology, 16(3): 117.
Steinman, H., Donson, H., Kawalski, M., Toerien, A. and Potter, P.C. 2003. Rising
prevalence of bronchial hyperresponsiveness and atopy in urban and rural South African
children. Asthma Acta, 4: 126-127.
Steinman, H., Donson, H., Kawalski, M., Toerien, A. and Potter, P.C. 2003. The rising
prevalence of bronchial hyperresponsiveness and atopy in urban and rural South African
children. World Asthma Congress, St. Petersburg, Russia. Asthma Acta, 4: 126.
Wessels, G., Hesseling, P.B., Rossouw, H.S., Wainwright, L., Poole, J., Stones, D. and
Harley, E.H. 2004. Epidemiological features of childhood leukaemia and other cancers in
South Africa. Pediatric Blood and Cancer, 43(4): 476/P.J.028.
EXTENSION AND DEVELOPMENT WORK

Artz, L. 2004 Member of Council, Criminology (and Victimology) Society of South Africa.
Artz, L. 2004. Editorial Board Member, Acta Criminologica.
Artz, L. 2004. Chairperson, Centre for the Study of Violence and Mental Health, Faculty of
Health Sciences, University of Cape Town.
Artz, L. 2004. Board Member, Family Law Centre, Cape Town
Artz, L. 2004. Gender Expert, [National] Alliance for Crime Prevention
Co-Convenor, Department of Justice/Lower Court Management Committee Domestic
Violence Act Working Group
Artz, L. 2004. Research Supervisor for SWEAT (Sex Workers Education and Advocacy
Taskforce)

Artz, L., Smythe, D. and Leggett, T. 2004. Reflections on integrated rape case management.
Pretoria: UNICEF, 1-102.
Moult, K. 2004. Justice served? Exploring alternative mechanisms to address violence
against women. Pretoria: UNICEF, 1-91.
The Problem of Attrition in Rape Cases, in News and Views for Magistrates (Cape Town:
Law, Race and Gender Research Unit, Faculty of Law, University of Cape Town), 1.
CONSULTANCY

Artz, L. 2004. Consultant to the Department of Justice on domestic violence courts and the
drafting of magistrate’s guidelines for the implementation of Domestic Violence Act.
Artz, L. 2004. Consultant to the Foundation for Human Rights and Social Surveys on a study
on the vulnerability of women in South Africa. Artz, L. 2004. Consultant to the Child Justice Alliance on research design for monitoring the implementation of the Child Justice Bill.
Artz, L. and Moult, K. 2004. Guest Course Convenors for Specific Offences (CRJ 512H):
Department of Criminal Justice, Faculty of Law, UCT.
Artz, L. and Smythe, D. 2004. Consultants to the National Directorate of Public Prosecutions
on sexual offences courts (attrition in rape cases).
Artz, L. and Smythe, D. 2004. Submissions to the South African Law Reform Commission on
Sexual Offences.
Doolan, K., Artz, L. and Smythe, D. 2004. Course lecturers for Sexual Assault, Domestic
Violence and the Law: Implications for Health Care Providers. Department of Obstetrics and
Gynaecology. Convenor: Professor Lynette Denny.
Maw, S. and Artz, L. 2004. Course lecturers for Understanding Gender. Department of
Obstetrics and Gynaecology. Course Convenor: Professor Lynette Denny.
Moult, K. 2004. Trainer and consultant to the Law, Race and Gender Research Unit on
domestic violence and HIV.
Smythe, D. and Artz, L. 2004. Consultants to the Open Society Foundation for review of
women’s advice centres in the Eastern Cape and KwaZulu-Natal
UNIVERSITY PUBLICATIONS AND WORKS OF A POPULAR NATURE

Artz, L. 2004. Guest Editorial: The Criminal Law (Sexual Offences) Amendment Bill. Journal
of Child and Adolescent Mental Health, 16(1): v-vii.
Kotwal, G.J. 2004. Review article. HIV treatment and eradication in South Africa. Journal of
Royal Society of Medicine, 97(1):1-2.
Kotwal, G.J. and Abrahams, M.R. 2004. Review article. Growing poxviruses and determining
virus titer. Methods in Molecular Biology, 269:101-12.
Potter, P.C. 2004. Sublingual allergy immunotherapy: Highly effective and safe in children
with allergic rhinitis and asthma. Paediatric Review, 1(1): 26-31.

Source: http://www.research2004.uct.ac.za/healthsci/data/clinlab.pdf