Microsoft word - dr. mukhopadhyay cv update 031813.doc

APARNA MUKHOPADHYAY, PhD
APPOINTMENTS:
March 2013- Present
Assistant Professor
Department of Physiology, Presidency University, Kolkata, India.
August 2012-December 2012
Adjunct Assistant Professor
Department of Biology, School of Science, Health and
Technology, Medgar Evers College, The City University of
New York, Brooklyn, NY, USA.
October 2007-November 2012
Research Associate
Department of Anatomy and Structural Biology and Marion
Bessin Liver Research Center, Albert Einstein College of
Medicine, Bronx, NY, USA.
May 2002-October 2007
Graduate Student
Department of Developmental and Molecular Biology,
Albert Einstein College of Medicine, Bronx, NY, USA.
September 2000-April 2002 Junior Research Fellow
Division of Molecular and Human Genetics, Indian Institute of Chemical Biology, Kolkata, India.

EDUCATION:

2007 PhD in Biomedical Sciences, Albert Einstein College of Medicine, Bronx, NY.
2004 MS with Distinction in Biomedical Sciences, Albert Einstein College of Medicine,
MSc in Biotechnology, Banasthali Vidyapith, Banasthali, India.
BSc in Physiology, Presidency College, Calcutta University, India

TEACHING EXPERIENCE:
March 2013-Present: Assistant Professor
Department of Physiology, Presidency University, Kolkata, India  Teach undergraduate and postgraduate students basic physiology, cell biology, genetics, molecular biology and microbiology.  Perform related administrative duties.  Apply for external funding and perform research activities for graduate and postgraduate
August 2012-December 2012: Adjunct Assistant Professor
Department of Biology, School of Science, Health and Technology, Medgar Evers College, The
City University of New York, Brooklyn
 Teach and supervise basic and advanced general biology laboratory courses (3 credit hours each) for students interested in a future in healthcare.  The courses are designed for science majors and include study of viruses, bacteria, protists, selected forms and functions of flowering plants, animal behavior and environment. June 2012-August 2012: Mentor an undergraduate student from the University of Arizona as
part of NIH funded Minorities Access to Research Careers (MARC) program and the Albert
Einstein College of Medicine Summer Undergraduate Research Program (SURP).
 Introduce basic concepts of cell biology and biomedical research as preparation to  Initiated over expression of sfGFP fusion proteins in HEK 293 cells for immunoprecipitation and identification of novel interacting partners for Rab1a.  Design and monitor progress of project and provide daily assistance and guidance.  Guide preparation of abstract and poster for presentation. 2011 Mentored a High school student from Yeshiva University High School for Girls in basic
hands-on laboratory techniques.
 Introduce basic concepts in cell biology, biochemistry and molecular biology.  Teach the basic concepts of biomedical research as an introduction to a career in  Teach basic laboratory techniques such as immunoblotting, immunoprecipitation and
2009 Responsible Conduct of Research Course, Group Discussion Leader.
 Facilitate discussion of ethic principles in a group of about 10 students.  Design and prepare questions related to topic. RESEARCH EXPERIENCE:
2007- 2012: Postdoctoral Researcher
Department of Anatomy and Structural Biology and Marion Bessin Liver Research Center,
Albert Einstein College of Medicine, Bronx, NY
Mentor: Dr. Allan Wolkoff
Partially Funded by NIH/NIDDK Hepatology Training Grant Fellowship (5T32DK007218-36)
 Initiated characterization of Rab1a’s role in endocytic processing in the liver.  Created siRNA mediated Rab1a knockdown cell lines to study endocytosis of fluorescent ligands by live cell imaging, microscopic examination of motility of endocytic vesicles on microtubules and motor recruitment, published in J. Cell Science in 2011.  Presented data in journal clubs, departmental meetings, domestic and international  Supervised two graduate, an undergraduate and a high school student in basic laboratory techniques and basic cell biology.  Generated data and wrote annual progress reports for NIH funded Training grant and

2002-2007: Doctoral training
Department of Developmental and Molecular Biology, Albert Einstein College of Medicine,
Bronx, NY
Mentor: Dr. Duncan Wilson
 Initiated investigation of membrane recruitment of Herpes Simplex Virus tegument  Generated a series of deletion mutants to discover a 42aa minimal region sufficient for targeting GFP into the virus particle, published in J. Virol, 2006.  Presented data at international, domestic and departmental meetings.  Trained and assisted three graduate students with their projects.
2000-2002: Junior Research Fellow
Division of Molecular and Human Genetics, Indian Institute of Chemical Biology, Kolkata, India.
Mentor: Dr. Keya Choudhury
 Initiated experiments to identify differentially expressed genes in radiation resistant strain M5 derived from the Chinese hamster V79 cell line, published in Radiation Research, 2003.  Developed and perfected quantitative PCR techniques to study the differential  Presented work and recent literature at journal clubs. 2000: Graduate Assistant
Banasthali Vidyapith, Banasthali, India.
Mentor: Dr. Binita Nautiyal
 Studied FSH, LH and PRL titers during puberty in mice by ELISA.  Maintained mice stock and monitored puberty in mice.  Prepared sections of testis and ovary for histological processing, sectioning and stain 1999: Summer Graduate Assistant
Bose Institute, Kolkata, India.
Mentor: Dr. Anuradha Lohia
 Acquired basic laboratory skills to clone, overexpress and purify E. histolytica ferrodoxin  Grew and cultured bacterial stocks  Prepared plasmid DNA without kits.
PUBLICATIONS:
A. Peer Reviewed Research Publications:
Mukhopadhyay A, Ashton AW, Lisanti MP, Machado FS, Weiss LM,
Mukherjee S. Microarray analysis of the mammalian thromboxane receptor-
Trypanosoma cruzi interaction. Cell Cycle. 2011 Apr 1;10(7):1132-43. Epub 2011 Apr 1.
2. Mukhopadhyay A, Nieves E, Che F, Wang J, Jin L, Murray JW, Gordon K, Angeletti
RH, Wolkoff AW. Proteomic Analysis of Endocytic Vesicles: I. Rab1a Regulates Motility
of Early Endocytic Vesicles. J Cell Sci. 2011 Mar 1;124(Pt 5):765-75. Epub 2011 Feb 8.
3. Mukherjee S, Nagajyothi F, Mukhopadhyay A, Machado FS, Belbin TJ, Campos de
Carvalho A, Guan F, Albanese C, Jelicks LA, Lisanti MP, Silva JS, Spray DC, Weiss LM,
Tanowitz HB. Alterations in myocardial gene expression associated with experimental
Trypanosoma cruzi infection. Genomics. 2008 May;91(5):423-32. Epub 2008 Mar 17.
4. Mukhopadhyay A, Lee GE, Wilson DW. The amino terminus of the herpes simplex virus
1 protein Vhs mediates membrane association and tegument incorporation. J Virol.
2006 Oct;80(20):10117-27.
5. Mukherjee S, Belbin TJ, Spray DC, Mukhopadhyay A, Nagajyothi F, Weiss LM,
Tanowitz HB. Microarray technology in the investigation of diseases of myocardium with
special reference to infection. Front Biosci. 2006 May 1;11:1802-13. Review.
6. Chi JH, Harley CA, Mukhopadhyay A, Wilson DW. The cytoplasmic tail of herpes
simplex virus envelope glycoprotein D binds to the tegument protein VP22 and to
capsids. J Gen Virol. 2005 Feb;86(Pt 2):253-61.
7. Chaudhuri K, Banerjee R, Pandit B, Mukherjee A, Das S, Sengupta S, Roychoudury S,
Bhattacharyya NP. Identification of two differentially expressed mitochondrial genes in a
methotrexate-resistant Chinese hamster cell strain derived from v79 cells using RNA
fingerprinting by arbitrary primed polymerase chain reaction. Radiat Res. 2003
Jul;160(1):77-85.
B. Contributions to International Conferences:

Oral Presentations:

1. Aparna Mukhopadhyay and Allan W. Wolkoff.
Rab1a ia a master regulator of early endocytic sorting events in hepatocytes. 63rd Annual Meeting of American Association for the Study of Liver Diseases, Nov 9 - 13, 2012; Boston, MA.
2. Aparna Mukhopadhyay and Allan W. Wolkoff.
Rab1a Regulates Minus End Directed Motility of Early Endocytic Vesicles. 61th
Annual meeting of the American association for the study of liver Diseases, Oct29-
Nov2, 2010, Boston, MA.

3. Aparna Mukhopadhyay, Ruth H. Angeletti, John W. Murray, and Allan W. Wolkoff.
Rab1a Regulates Motility and Trafficking of Early Endocytic Vesicles. 60th Annual meeting of the American association for the study of liver Diseases, Oct30-Nov3, 2009, Boston, MA.
Abstracts/Posters at International Conferences:

1. Aparna Mukhopadhyay and Allan W. Wolkoff.
Rab1a is a global regulator of endocytosis via clathrin coated vesicles. 62th Annual meeting of the American Association for the Study of Liver Diseases, Nov 4-8, 2011 San Francisco, CA.
2. Aparna
Mukhopadhyay, John W Murray and Allan W Wolkoff
Characterizing the role of Rab1a in Endocytic Trafficking. 48th Annual Meeting for the American Society for Cell Biology, Dec 13-17, 2008, San Francisco, CA. 3. DW Wilson and Mukhopadhyay A.
The Amino terminus of the Herpes Simplex Virus protein Vhs mediates Membrane
Association and Tegument Incorporation. 31st International Herpesvirus Workshop,
July 22-28, 2006. University of Washington, Seattle, WA.

4. Mukhopadhyay,
Identification of tegument targeting sequence in HSV-1. 10th Annual Julius Marmur Symposium, March 22, 2006. Albert Einstein College of Medicine, Bronx, NY- 10461.
AWARDS AND HONORS:
2011: Awarded Presidential Poster of Distinction at the 62th Annual meeting of the American Association for the Study of Liver Diseases, Nov 4-8, 2011 San Francisco, CA. 2004: Passed MS with Distinction at the Albert Einstein College of Medicine, Bronx, New 2001: Honored for obtaining highest marks in the PhD qualifying Examination organized by Indian Institute of Chemical Biology, Kolkata, India. 1999: Awarded Junior Research fellowship (CSIR) through a competitive National Level Eligibility (NET) test conducted jointly by Council of Scientific and Industrial Research (CSIR) and University Grants Commission (UGC) in 1999, Govt. of India. 1997: Received first prize for scoring the highest marks in BSc Physiology, Part-1 examination in 1997, University of Calcutta (Kolkata), India.
PARTICIPATION IN NIH FUNDED RESEARCH:
2007-2010: Research funded by NIH/NIDDK Hepatology Training Grant (5T32 DK007218-36).

PROFESSIONAL AFFILIATIONS:

1. Member of American Society of Cell Biologist (ASCB) from 2008. 2. Member of New York Academy of Sciences (NYAS) from 2006.
PROFESSIONAL SKILLS:

 Animal Manipulations: Mouse eye bleeding.
 Cell culture: Culture of numerous cell lines, generation of stable transfectants.
 Histology: Histological and histochemical techniques, paraffin embedded tissue sections.
 Cellular Sub-fractionation: Subcellular and suborganeller fractionation using continuous
and discontinuous gradients, preparations of cellular membranes and endocytic vesicles.  Molecular Biology: Preparation and purification of genomic and plasmid DNA, extraction of
RNA and proteins, cloning in bacteria, in vitro transcription and translation, arbitary primed PCR for Differential Display, Enzyme Linked Immunosorbant Assay (ELISA) and standard techniques.  Virology: Propagation of Viruses in cell culture, preparation of post-nuclear supernatants
and infected cell lysates, plaque assay, purification of extracellular virions.  siRNA: design of siRNAs, generation of stable knockdown cells including tet-inducible lines
 Microscopy: Preparation of fluorescent microtubules, generation of polarity marked
microtubules, vesicle motility assays, staining of cell and vesicles by immunofluorescence, live cell imaging. Familiar with use and image acquisition in spinning disc confocal and Olympus 1x71 inverted microscopes for fixed and live cell imaging, transmission electron microscopy including use of Qdots and immunogold labeling.  Protein Analysis: Mass spectrometric analysis, Preparation of soluble S-100 extract,
purification, concentration and dialysis of proteins, in vitro translation, immunoprecipitation with protein A/G agarose beads, binding assays using GST fusions. fluorographic and electro chemiluminescent (ECL) detection of proteins.  Computer proficiency: Image analysis and processing using Metamorph, Adobe
Photoshop and Image J, creation of reference databases with Endnote, preparation and presentation of data using scientific slides and working with Internet and Databases.
CITIZENSHIP: Indian
US IMMIGRATION STATUS: Permanent Resident

REFERENCES:
Dr. Allan W. Wolkoff
Director, Marion Bessin Liver research Center and Professor, Department of Anatomy and
Structural Biology
Albert Einstein College of Medicine
Jack and Pearl Resnick Campus
1300 Morris Park Avenue
Ullmann Building, Room 517
Bronx, NY 10461
Phone: 718.430.3798
[email protected]
Dr. Duncan Wilson
Professor, Department of Developmental and Molecular Biology
Albert Einstein College of Medicine
Jack and Pearl Resnick Campus
1300 Morris Park Avenue
Chanin Building, Room 513
Bronx, NY 10461
Phone: 718.430.2305
[email protected]

Dr. Anthony Udeogalanya
Professor and Chair, Department of Biology, School of Science, Health and Technology
Medgar Evers College-CUNY
1638 Bedford Avenue
Brooklyn, NY-11225
Phone: (718) 270-6200
Fax: (718) 270-6498
[email protected]

Source: http://presiuniv.ac.in/staff/staffcv/1367559357__Dr.%20Mukhopadhyay%20CV%20update%20031813.pdf